Microliter scale solid phase extraction devices
First Claim
1. A device for microliter scale treatment of fluid samples, comprising, a central containment member having a first containment bore disposed therethrough with openings at input and output ends of the first containment bore and where the input and output ends of the first containment bore are surrounded by first and second sealing surfaces adjacent to the input and output ends, respectively, a first conduit assembly having a first conduit bore disposed therethrough with a first opening of the first conduit being surrounded by a third sealing surface adjacent to the first opening;
- a second conduit assembly having an second conduit bore disposed therethrough with an second opening of the second conduit being surrounded by a fourth sealing surface adjacent to the second opening;
a first porous membrane sealingly engaged between the first sealing surface of the first containment bore and the third sealing surface of the first conduit assembly so that a perimeter of the first porous membrane is sealed from fluid flow while a first fluid contact area of the first porous membrane is disposed between the input opening of the containment bore and the first opening of the first conduit; and
a second porous membrane sealingly engaged between the second sealing surface of the first containment bore and the fourth sealing surface of the second conduit assembly so that a perimeter of the second porous membrane is sealed from fluid flow while a second fluid contact area of the second porous membrane is disposed between the output opening of the containment bore and the second opening of the second conduit, and the volume of the containment bore is enclosed at the input and output ends by the first and second fluid contact areas of the first and second porous membranes.
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Accused Products
Abstract
Microliter scale solid phase extraction devices for preparing analytes in microliter volumes are disclosed. A re-useable device is provided in cartridge form that includes a central containment member with a containment bore that holds as little as 1 to 5 μl or less of a solid phase material that binds the analyte. The containment bore is enclosed on either side by a porous membrane that has an inner portion exposed for fluid flow and a peripheral portion that is sealed against fluid flow. The seal is formed by engaging the periphery of the porous membranes between sealing surfaces of the central containment member and corresponding sealing surfaces on first and second conduit assemblies that comprise the remainder of the cartridge. A non-reuseable device is provided in “chip” form, which includes a porous filter sandwiched between top and bottom wafers each having a plurality corresponding input and output conduits for a plurality of samples.
63 Citations
64 Claims
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1. A device for microliter scale treatment of fluid samples, comprising,
a central containment member having a first containment bore disposed therethrough with openings at input and output ends of the first containment bore and where the input and output ends of the first containment bore are surrounded by first and second sealing surfaces adjacent to the input and output ends, respectively, a first conduit assembly having a first conduit bore disposed therethrough with a first opening of the first conduit being surrounded by a third sealing surface adjacent to the first opening; -
a second conduit assembly having an second conduit bore disposed therethrough with an second opening of the second conduit being surrounded by a fourth sealing surface adjacent to the second opening;
a first porous membrane sealingly engaged between the first sealing surface of the first containment bore and the third sealing surface of the first conduit assembly so that a perimeter of the first porous membrane is sealed from fluid flow while a first fluid contact area of the first porous membrane is disposed between the input opening of the containment bore and the first opening of the first conduit; and
a second porous membrane sealingly engaged between the second sealing surface of the first containment bore and the fourth sealing surface of the second conduit assembly so that a perimeter of the second porous membrane is sealed from fluid flow while a second fluid contact area of the second porous membrane is disposed between the output opening of the containment bore and the second opening of the second conduit, and the volume of the containment bore is enclosed at the input and output ends by the first and second fluid contact areas of the first and second porous membranes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 55, 56, 57, 58)
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23. A device for microliter scale treatment of samples, comprising,
a first planar wafer having an input conduit bore with a first longitudinal axis longitudinally disposed in and transverse to, a first plane of the first wafer, the input conduit bore having an input end and an output end with a first sealing surface surrounding the output end of the input bore; -
a second planar wafer having output conduit bore with a second longitudinal axis longitudinally disposed in and transverse to, a second plane of the second wafer, the output conduit bore having an input end and an output end with a second sealing surface surrounding the output end of the input bore;
a third planar wafer sandwiched between the first and second wafers, the third planar wafer having a containment bore longitudinally disposed in and transverse to, a third plane of the third wafer, the containment bore having first and second openings at either end, each opening being surrounded by a third and fourth sealing surfaces;
a first porous membrane sealingly engaged between the first sealing surface of the first wafer and the third sealing surfaces of the third wafer so that a perimeter of the first porous membrane is sealed from fluid flow while a first fluid contact area of the first porous membrane is disposed between one opening of the containment bore and one of the output openings the first wafer; and
a second porous membrane sealingly engaged between the second sealing surface of the second wafer and the fourth sealing surfaces of the third wafer so that a perimeter of the second porous membrane is sealed from fluid flow while a second fluid contact area of the second porous membrane is disposed between the other opening of the containment bore and the input opening the second wafer, thereby enclosing a volume of the containment bore between the first and second porous membranes. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 49, 50, 51, 52, 53, 54, 59, 60, 61, 62, 63, 64)
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Specification