Methods and compositions for analysis of regulatory sequences
First Claim
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1. A method for making an array, the method comprising:
- (a) isolating a plurality of cellular polynucleotide sequences, whereby the sequences are isolated based on their accessibility in cellular chromatin; and
(b) attaching each of the isolated sequences to an address on a solid support.
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Abstract
Methods for constructing arrays of regulatory sequences, and the arrays so obtained, are provided. Regulatory sequences for use on the arrays are isolated based on their accessibility in cellular chromatin. A number of methods for using the arrays are disclosed, including regulatory DNA profiling, epigenome profiling, toxicological profiling and identification of in vivo binding sites of DNA binding proteins in complex genomes.
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15 Claims
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1. A method for making an array, the method comprising:
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(a) isolating a plurality of cellular polynucleotide sequences, whereby the sequences are isolated based on their accessibility in cellular chromatin; and
(b) attaching each of the isolated sequences to an address on a solid support.
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2. An array comprising a plurality of accessible polynucleotide sequences, wherein:
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(a) the sequences are isolated based on their accessibility in cellular chromatin; and
(b) each accessible sequence is located at a distinct address on a solid support. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification