Method and apparatus for processing polynucleotide-containing samples
First Claim
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1. A sample preparation device, comprising:
- a surface comprising a poly-cationic polyamide bound thereto; and
a sample introduction passage in communication with the surface for contacting the surface with a fluidic sample.
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Abstract
Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
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Citations
23 Claims
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1. A sample preparation device, comprising:
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a surface comprising a poly-cationic polyamide bound thereto; and
a sample introduction passage in communication with the surface for contacting the surface with a fluidic sample. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for processing a sample, comprising:
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providing a mixture comprising a liquid and an amount of polynucleotide;
contacting a retention member with the mixture, the retention member configured to preferentially retain polynucleotides as compared to polymerase chain reaction inhibitors;
separating substantially all of the liquid in the mixture from the retention member; and
releasing polynucleotides retained by the retention member therefrom. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification