Sample preparation devices and methods
First Claim
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1. A sample preparation method, the method comprising:
- providing a biological sample, wherein the biological sample comprises microorganisms;
providing a housing to lyse the microorganisms and separate biological material contained in the microorganisms, wherein the housing comprises a separation membrane to separate the biological material contained in the microorganisms;
providing a lysis buffer to lyse the microorganisms;
lysing the microorganisms to release the biological material and form a lysate;
collecting the biological material.
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Abstract
A method and device for preparing biological samples includes lysing the microorganisms, and collecting a desired biological material.
69 Citations
20 Claims
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1. A sample preparation method, the method comprising:
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providing a biological sample, wherein the biological sample comprises microorganisms;
providing a housing to lyse the microorganisms and separate biological material contained in the microorganisms, wherein the housing comprises a separation membrane to separate the biological material contained in the microorganisms;
providing a lysis buffer to lyse the microorganisms;
lysing the microorganisms to release the biological material and form a lysate;
collecting the biological material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A sample preparation method, the method comprising:
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providing a housing configured to receive a biological sample, the housing comprising;
a first chamber configured to mix a lysis buffer and the biological sample to form a lysate;
a filtering mechanism configured to retain a biological material from the lysate;
a second chamber configured to contain an eluting buffer, the second chamber being configured to be selectively placed in flow communication with the filtering mechanism to flow the eluting buffer to the filtering mechanism to elute the biological material from the filtering mechanism; and
providing a flow member configured to provide force within the housing to cause liquid flow relative to the housing providing the biological sample to the first chamber, wherein the biological sample comprises microorganisms;
lysing the microorganisms to release the biological material and form a lysate; and
eluting the biological material from the filtering mechanism. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
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20. A method for preparing a biological sample, the method comprising:
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mixing a biological sample with a lysing buffer to lyse microorganisms in the biological sample and create a lysate; and
flowing the lysate to a filtering mechanism configured to pass biological material in the lysate and capture other material in the lysate, wherein the mixing and flowing are performed within a multi-chambered syringe housing.
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Specification