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Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same

  • US 20060166361A1
  • Filed: 12/08/2005
  • Published: 07/27/2006
  • Est. Priority Date: 12/21/2004
  • Status: Abandoned Application
First Claim
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1. A method for forming cell sheets, which comprises coating a support with a polymer or copolymer which has a lower or upper critical solution temperature within the range of 0°

  • C. to 80°

    C., culturing cells attached to the surface thereof at a higher temperature than said lower critical solution temperature or at a lower temperature than said upper critical solution temperature on said coated surface, and detaching and collecting said cell sheet from said coated surface by changing the temperature to a lower temperature than said lower critical solution temperature or at a higher temperature than said upper critical solution temperature, wherein said cells are placenta-derived cells comprising cells derived from human postpartum placenta tissue substantially free of blood, wherein said cells self-renew and expand in culture, wherein said cells are multipotent, and wherein said cells grow in about 5% to about 20% oxygen.

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