Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same

• US 20060166361A1
• Filed: 12/08/2005
• Published: 07/27/2006
• Est. Priority Date: 12/21/2004
• Status: Abandoned Application

• Current Assignee: Ethicon Incorporated (Johnson & Johnson)
• Original Assignee: Ethicon Incorporated (Johnson & Johnson)
• Inventors: Gosiewska, Anna, Seyda, Agnieszka
• Application Number:
  US11/297,778
• Publication Number:
  US 20060166361A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: C12N 2502/02 embryonic cells
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2506/03 from non-embryonic pluripotent stem cells
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 2539/10 Coating allowing for selective detachment of cells, e.g. thermoreactive coating
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: C12N 5/0607 Non-embryonic pluripotent stem cells, e.g. MASC induced pluripotent stem cells C12N5/0696

Abstract

Cells derived from postpartum placenta and methods for their isolation are provided by the invention. The invention further provides cultures and compositions of the placenta-derived cells. The placenta-derived cells of the invention have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications.

Claims

1. A method for forming cell sheets, which comprises coating a support with a polymer or copolymer which has a lower or upper critical solution temperature within the range of 0°

C. to 80°

C., culturing cells attached to the surface thereof at a higher temperature than said lower critical solution temperature or at a lower temperature than said upper critical solution temperature on said coated surface, and detaching and collecting said cell sheet from said coated surface by changing the temperature to a lower temperature than said lower critical solution temperature or at a higher temperature than said upper critical solution temperature, wherein said cells are placenta-derived cells comprising cells derived from human postpartum placenta tissue substantially free of blood, wherein said cells self-renew and expand in culture, wherein said cells are multipotent, and wherein said cells grow in about 5% to about 20% oxygen.

2. The method of claim 1 wherein said polymer or copolymer comprises the reaction products of monomers selected from the group consisting of acrylamide, methacrylamide, N-ethyl acrylamide, N-n-propyl acrylamide, N-n-propyl meihacrylamide, N-isopropyl acrylamide, N-isopropyl methacrylamide, N-cyclopropyl acrylamide, N-cyclopropyl methacrylamide, N-ethoxyethyl acrylamide, N-ethoxyethyl methacrylamide, N-tetrahydrofurfuryl acrylamide, N- tetrahydrofurfuryl methacrylamide, N,N-dimethyl (meth)acrylamide, N,N-ethylmethyl acrylamide, N,N-diethyl acrylamide), 1-(1-oxo-2-propenyl)-pyrrolidine, 1-(1-oxo-2-propenyl)-piperidine, 4-(1-oxo-2-propenyl)-morpholine, 1-(1-oxo-2-methyl-2-propenyl)-pyrrolidine, 1-(1-oxo-2-methyl-2-propenyl)-piperidine, 4-(1-oxo-2-methyl-2-propenyl)-morpholine, and methyl vinyl ether.

3. The method of claim 2 wherein said polymer comprises the reaction product of N-isopropyl acrylamide.

4. The method of claim 1 wherein said support is comprised of a material selected from the group consisting of polystyrene, poly(methyl methacrylate)), polypropylene, polyethylene, vinyl polymers, ceramics, metals, glass and modified glass.

5. The method of claim 1 wherein said cells are comprised of a co-culture comprised of isolated placenta-derived cells comprising cells derived from mammalian placenta tissue substantially free of blood and another mammalian cell of any phenotype.

6. The method of claim 5 wherein said another mammalian cell comprise a human cell line.

7. A method for forming sheets of isolated placenta-derived cells, which comprises coating a support with a polymer which has a lower critical solution temperature of less than about 30°

C., culturing said cells at 37°

C. to confluence, lowering the temperature to 20°

C., and collecting said sheets.

8. The method of claim 7 wherein said isolated umbilicus-derived cells comprise cells derived from human postpartum placenta tissue substantially free of blood, wherein said cells self-renew and expand in culture;

wherein said cells are multipotent;

wherein said cells require L-valine for growth;

wherein said cells grow in about 5% to about 20% oxygen.

9. The method of claim 7 wherein said polymer comprises the reaction product of N-isopropyl acrylamide.

10. The method of claim 7 wherein said support is comprised of a material selected from the group consisting of polystyrene, poly(methyl methacrylate)), ceramics, metals, glass and modified glass.

11. A method for therapeutically treating mammalian tissue, said method comprising the steps of

I. providing a cell sheet, the cell sheet formed by a method which comprises coating a support with a polymer or copolymer which.has a lower or upper critical solution temperature within the range of 0°

C. to 80°

C., culturing cells attached to the surface thereof at a higher temperature than said lower critical solution temperature or at a lower temperature than said upper critical solution temperature on said coated surface, and detaching and collecting said cell sheet from said coated surface by changing the temperature to a lower temperature than said lower critical solution temperature or at a higher temperature than said upper critical solution temperature,wherein said cells are placenta-derived cells comprising cells derived from human postpartum placenta tissue substantially free of blood, wherein said cells self-renew and expand in culture, wherein said cells are multipotent, wherein said cells require L-valine for growth, and wherein said cells grow in about 5% to about 20% oxygen; and

,

II. transplanting the cell sheet to mammalian tissue.

12. The method of claim 11 wherein said polymer or copolymer comprises the reaction products of monomers selected from the group consisting of acrylamide, methacrylamide, N-ethyl acrylamide, N-n-propyl acrylamide, N-n-propyl methacrylamide, N-isopropyl acrylamide, N-isopropyl methacrylamide, N-cyclopropyl acrylamide, N-cyclopropyl methacrylamide, N-ethoxyethyl acrylamide, N-ethoxyethyl methacrylamide, N-tetrahydrofurfuryl acrylamide, N-tetrahydrofurfuryl methacrylamide, N,N-dimethyl (meth)acrylamide, N,N-ethylmethyl acrylamide, N,N-diethyl acrylamide), 1-(1-oxo-2-propenyl)-pyrrolidine, 1-(1-oxo-2-propenyl)-piperidine, 4-(1-oxo-2-propenyl)-morpholine, 1-(1-oxo-2-methyl-2-propenyl)-pyrrolidine, 1-(1-oxo-2-methyl-2-propenyl)-piperidine, 4-(1-oxo-2-methyl72-propenyl)-morpholine, and methyl vinyl ether.