Microscale diffusion immunoassay utilizing multivalent reactants

• US 20060166375A1
• Filed: 09/14/2005
• Published: 07/27/2006
• Est. Priority Date: 09/23/2004
• Status: Active Grant

• Current Assignee: University of Washington
• Original Assignee: University of Washington
• Inventors: Yager, Paul, Hawkins, Kenneth R.
• Granted Patent: US 7,550,267 B2
• Time in Patent Office: Days
• US Class Current: 436/524
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An improved microscale diffusion immunoassay utilizing multivalent reactants is disclosed. In particular, a method for detecting the presence of analyte particles in an analyte fluid is disclosed, the method comprising: (a) providing the analyte fluid comprising the analyte particles; (b) providing a diffusion fluid comprising binding particles capable of binding with the analyte particles; (c) flowing the analyte fluid and the diffusion fluid in adjacent laminar flow through a microfluidic channel; (d) allowing the analyte particles to diffuse into the diffusion fluid and bind with the binding particles to form analyte/binding particle complexes; and (e) detecting the presence of the analyte particles and the analyte/binding particle complexes, wherein each of the binding particles is capable of binding with more than one analyte particle, and wherein each of the analyte particles is capable of binding with more than one binding particle. Methods for determining the concentration of the analyte particles are also disclosed.

Claims

1. A method for detecting the presence of analyte particles in an analyte fluid comprising:

(a) providing the analyte fluid comprising the analyte particles;

(b) providing a diffusion fluid comprising binding particles capable of binding with the analyte particles;

(c) flowing the analyte fluid and the diffusion fluid in adjacent laminar flow through a microfluidic channel;

(d) allowing the analyte particles to diffuse into the diffusion fluid and bind with the binding particles to form analyte/binding particle complexes; and

(e) detecting the presence of the analyte particles and the analyte/binding particle complexes,

wherein each of the binding particles is capable of binding with more than one analyte particle, and wherein each of the analyte particles is capable of binding with more than one binding particle.

2. The method of claim 1, further comprising:

(f) detecting a diffusion profile in the microfluidic channel formed by the analyte particles and the analyte/binding particle complexes; and

(g) determining the concentration of the analyte particles.

3. The method of claim 2 wherein the concentration of analyte particles is determined based upon the diffusion profile of the analyte particles and analyte/binding particle complexes.

4. The method of claim 2 wherein the concentration of analyte particles is determined by comparing the diffusion profile to a calibration profile.

5. The method of claim 1 wherein at least one of the analyte particles is labeled with a detectable marker.

6. The method of claim 1 wherein at least one of the binding particles is labeled with a detectable marker.

7. The method of claim 1 wherein the binding particles are antibodies.