Liquid sampling utilizing ribbed pipette tip for barrier penetration

• US 20060171851A1
• Filed: 01/28/2005
• Published: 08/03/2006
• Est. Priority Date: 01/28/2005
• Status: Abandoned Application

• Current Assignee: Molecular BioProducts Incorporated (Perspecta, Inc.)
• Original Assignee: Molecular BioProducts Incorporated (Perspecta, Inc.)
• Inventors: Motadel, Arta
• Application Number:
  US11/045,530
• Publication Number:
  US 20060171851A1
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips

Abstract

An apparatus and method for liquid sampling utilizing a ribbed pipette tip for barrier penetration is disclosed herein. The unique, disposable plastic pipette tip of the present invention has an outer surface with at least three ribs extending longitudinally along the outer surface of the barrel. Each rib is circumferentially spaced from one another at a uniform distance, with each rib being symmetrically sized and positioned on the pipette tip barrel. The pipette tip is capable of being placed on a mounting shaft of a hand-held pipette or on the mounting head of an automated liquid handling machine. The pipette tip is rigid and straight enough to pierce a barrier sheet or resilient barrier sealing a container holding a liquid to be sampled. The ribs of the pipette tip operate to keep the barrier separated from the outer surface of the pipette tip such that ambient air is allowed to flow into and from the interior of the sealed container assembly during aspiration of the liquid sample into the pipette tip allowing for accurate transfer of liquids while minimizing the risk of contamination. The pipette tip and method of the present invention may be utilized in an automated pipetting system to sample an array of sealed containers, such as sealed wells and the pipette tip is sufficiently. The pipette of the present invention may include a self-sealing filter to prevent cross-contamination.

Claims

1. A disposable plastic pipette tip comprising a hollow body having a proximal opening at its proximal end for mounting to a mounting shaft of a pipettor and a distal opening at its distal end for aspirating liquid into and dispensing liquid from the hollow body, the pipette tip comprising:

a collar encircling and extending from the proximal opening; and

a barrel extending from the collar to the distal opening, the barrel having an outer surface with at least four ribs extending longitudinally along the outside surface of the barrel, each rib being circumferentially spaced from one another at a uniform distance from each other and each rib being symmetrically sized and positioned on the pipette tip barrel; and

wherein a central longitudinal axis passes through the hollow body of the pipette tip, each rib has an apex, and the distance from the central longitudinal axis to the apex of each rib is such that an imaginary line passing through the apex to an adjacent apex in a plane perpendicular to the central longitudinal axis does not otherwise intersect the outer surface of the pipette tip barrel.

2. A pipette tip as recited in claim 1 wherein an edge of the barrel defining the distal opening lies in a plane perpendicular to the central longitudinal axis.

3. A pipette tip as recited in claim 1 wherein the barrel has an upper barrel portion adjacent the collar and a lower barrel portion adjacent the distal opening, the upper barrel portion being in the shape of a truncated cone, the lower barrel portion being in the shape of an elongated cone in which the diameter of the hollow body tapers to be smaller at a lower rate with respect to longitudinal central axis than the upper barrel portion, and each rib extends longitudinally along the barrel from the lower portion to the upper portion.

4. The pipette tip recited in claim 1 wherein each rib extends longitudinally completely to the distal opening.

5. The pipette tip as recited in claim 4 wherein the thickness of each rib as measured away from the outer barrel surface tapers as it approaches the distal opening so that the thickness of each respective rib from the barrel surface converges to zero as the rib approaches the edge of the barrel defining the distal opening.

6. The pipette tip as recited in claim 3 wherein the thickness of each rib away from the outer barrel surface lessens and converges to zero as it extends along the upper barrel portion towards the collar.

7. The pipette tip recited in claim 1 wherein a central longitudinal axis passes through the hollow body of the pipette tip and at least a portion of an outer surface of the collar extends along the hollow body from the proximal opening towards the distal opening until reaching a shoulder that connects the outer surface of the collar to the outer surface of the barrel, the shoulder being substantially perpendicular to the longitudinal central axis.

8. The pipette tip as recited in claim 1 wherein the pipette tip further comprises a filter located in the hollow body of the pipette tip.

9. The pipette tip as recited in claim 8 wherein the filter is a self-sealing filter.

10. A method of sampling a liquid sample from a sealed container assembly using a disposable, plastic pipette tip, the method comprising the steps of:

providing a liquid sample in a sealed container assembly comprising a container having a closed bottom portion and a top portion with an open end, the liquid sample being contained in the bottom portion of the container, and a closure associated with the top portion of the container that seals the open end of the container, the closure comprising at least in part a resilient barrier that separates an interior of the container from the ambient environment;

a) providing a disposable, plastic pipette tip comprising a hollow body having a proximal opening at its proximal end for mounting to a mounting shaft of a pipettor and a distal opening at its distal end for aspirating liquid into and dispensing liquid from the hollow body, the hollow body comprising a collar encircling and extending from the proximal opening, and a barrel extending from the collar to the distal opening, the barrel having an outer surface with at least four ribs extending longitudinally along the outside surface of the barrel;

b) placing the recited pipette tip on a mounting shaft of a pipettor;

piercing the resilient barrier on the container assembly closure with the distal end of the pipette tip barrel and moving the barrel through the resilient barrier until the distal opening is submerged in the liquid sample in the sealed container assembly;

d) aspirating liquid sample from the container into the pipette tip through the submerged distal opening;

e) while aspirating the liquid specimen into the pipette tip, using the ribs on the pipette tip barrel to spread the pierced opening in the resilient barrier such that ambient air is able to flow into and from the interior of the sealed container assembly during aspiration of the liquid sample into the pipette tip; and

f) removing the pipette tip containing the aspirated liquid sample from the container assembly.

11. The method recited in claim 10 wherein the liquid sample is a patient specimen.

12. The method recited in claim 10 wherein the pipette tip further comprises a filter located in the hollow body of the pipette tip.

13. The method recited in claim 12 wherein the filter is a self-sealing filter.

14. The method recited in claim 10 wherein the recited container assembly is a patient specimen tube and the recited closure is a screw cap having an opening through its top that is covered by a resilient, automatically resealing septum, and wherein the step of removing the pipette tip further includes allowing the pierced opening in the resilient automatically resealing member to close.

15. The method recited in claim 10 wherein the recited container assembly is a patient specimen tube and the recited closure is an automatically resealing elastomeric plug-type stopper, and wherein the step of removing the pipette tip further includes allowing the pierced opening in the resilient automatically resealing septum to close.

16. The method recited in claim 10 wherein the recited container assembly is a cluster tube and the recited closure is an automatically resealing elastomeric plug-type stopper, and wherein the step of removing the pipette tip further includes allowing the pierced opening in the resilient automatically resealing member to close.

17. The method recited in claim 10 wherein the recited container assembly is a well in a well plate having an array of wells and the recited closure is an automatically resealing elastomeric plug-type stopper, and wherein the step of removing the pipette tip further includes allowing the pierced opening in the resilient automatically resealing member to close.

18. A method of sampling a plurality of liquid samples each contained in one of a plurality of sealed container assemblies with a plurality of disposable, plastic pipette tips, the method comprising the steps of:

a) providing a plurality of liquid samples each in a sealed container assembly, the sealed container assemblies being arranged in columns and rows to form a two dimensional array, each sealed container assembly comprising a container having a closed bottom portion and a top portion with an open end, the respective liquid sample being contained in the bottom portion of the respective container, and a closure associated with the top portion of each container and sealing its open end, the closure comprising at least in part a resilient barrier that separates an interior of the container from the ambient environment;

b) providing a plurality of disposable, plastic pipette tips each comprising a hollow body having a proximal opening at its proximal end configured to be mounted on the mounting shaft of an automated pipetting system configured to accept an array of pipette tips and a distal opening at its distal end for aspirating liquid into and dispensing liquid from the hollow body, the hollow body comprising a collar encircling and extending from the proximal opening, a barrel extending from the collar to the distal opening, the barrel having an outer surface with at least four ribs extending longitudinally along the outside surface of the barrel;

c) mounting a plurality of the recited pipette tips in an array on the mounting head for an automated pipetting system;

d) contemporaneously piercing at least some of the resilient barriers on the array of container assemblies with the distal end of at least some of the mounted pipette tips and moving the barrel of the pipette tips through the respective resilient barriers until the tip openings at the distal ends of the pipette tips are submerged in the liquid samples in the respective sealed container assemblies;

e) aspirating the liquid samples into the respective pipette tips through the submerged distal openings;

f) while aspirating the liquid specimens into the respective pipette tip, using the ribs on the pipette tip barrel to spread the pierced opening in the respective resilient barrier such that ambient air is able to flow into and from the interior of the respective sealed container assembly during aspiration of the sample into the pipette tip; and

g) removing each respective pipette tip containing aspirated liquid sample from the respective container assembly.

19. The method recited in claim 18 wherein the recited container is a cluster tube that is placed into a tube rack along with other cluster tubes to form an array of cluster tubes in the rack;

and the recited closure is an automatically resealing elastomeric plug-type stopper, and wherein the step of removing each respective pipette tip further includes allowing the pierced opening in the resilient automatically resealing member to close.

20. The method recited in claim 18 wherein the recited container is a well in a well plate having an array of wells, and a recited closure is an automatically resealing elastomeric plug-type closure, and wherein the step of removing each respective pipette tip further includes allowing the pierced opening in the resilient automatically resealing member to close.

21. The method recited in claim 18 wherein the recited container is a well in a well plate having an array of wells, and a recited closure is an automatically resealing elastomeric plug-type closure that is formed together with other such plug-type closures in an array integral with a sealing mat, and wherein the step of removing each respective pipette tip further includes allowing the pierced opening in the resilient automatically resealing member to close.

22. The method recited in claim 18 wherein the plurality of disposable plastic pipette tips are provided in an array in a rack prior to mounting the pipette tips in an array on the mounting head for the automated pipetting system.