Methods for evaluating the activity of candidate agents

• US 20060171890A1
• Filed: 01/27/2006
• Published: 08/03/2006
• Est. Priority Date: 01/28/2005
• Status: Abandoned Application

• Current Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Original Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Inventors: Yeomans, David C., Angst, Martin, Peters, Mary, Peters, Michael C.
• Application Number:
  US11/342,246
• Publication Number:
  US 20060171890A1
• Time in Patent Office: Days
• US Class Current: 424/9.200
• CPC Class Codes: A61K 49/0008 Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure

Abstract

Methods for evaluating the activity of candidate agents are provided. In practicing the subject methods, a candidate agent is administered to an injured tissue site of an animal and the activity of the candidate agent on the injured tissue site is evaluated. Also provided are kits and systems for use in practicing the subject methods.

Claims

1. A method for evaluating the activity of a candidate anti-inflammatory, local anesthetic, or analgesic agent, said method comprising:

locally or systemically administering said candidate agent to a normal or injured cutaneous, joint, or muscular tissue site of an animal; and

assessing said injured tissue site to evaluate anti-inflammatory, local anesthetic, or analgesic activity of said candidate agents.

2. The method according to claim 1, wherein said candidate agent is locally administered by microdialysis.

3. The method according to claim 1, wherein said method further comprises injuring said tissue site prior to said locally administering step.

4. The method according to claim 3, wherein said injuring comprises physically injuring said tissue site.

5. The method according to claim 3, wherein said injuring comprises chemically injuring said tissue site.

6. The method according to claim 1, wherein said animal is a mammal.

7. The method according to claim 6, wherein said mammal is a human.

8. The method according to claim 1, wherein said candidate analgesic agent is a small molecule.

9. The method according to claim 1, wherein said assessing comprises assaying for the release of at least one injury mediating chemical from said site.

10. The method according to claim 9, wherein said at least one injury mediating chemical is a prostaglandin.

11. The method according to claim 1, wherein said assessing comprises measuring vascular perfusion at said site.

12. The method according to claim 11, wherein vascular perfusion at said site is measured by Scanning Laser Doppler Imaging.

13. The method according to claim 1, wherein said assessing comprises physical inspection of said injured tissue site.

14. The method according to claim 1, wherein said assessing comprises subjectively interrogating said animal.

15. A kit comprising:

a local administration element for locally administering a candidate agent to a tissue site; and

instructions for using said element in a method of evaluating the activity of a candidate agent.

16. The kit according to claim 15, wherein said local administration element is a microdialysis device.

17. The kit according to claim 15, wherein said kit further comprises a tissue site injury element.

18. The kit according to claim 17, wherein said injury element is a physical injury element.

19. The kit according to claim 17, wherein said injury element is a chemical injury element.

20. The kit according to claim 15, wherein said kit further comprises a cutaneous tissue site injury assessment element.

21. A system comprising:

(a) a candidate gent;

(b) an element for locally administering said candidate agent to a tissue site; and

(c) an animal.

22. The system according to claim 21, wherein said local administration element is a microdialysis device.

23. The system according to claim 22, wherein said system further comprises a tissue site injury element.

24. The system according to claim 23, wherein said injury element is a physical injury element.

25. The system according to claim 23, wherein said injury element is a chemical injury element.

26. The system according to claim 21, wherein said system further comprises a tissue site injury assessment element.