Sequencing of surface immobilized polymers utilizing microflourescence detection
First Claim
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1. A method for determining the incorporation of nucleotides comprising:
- (a) providing a nucleic acid array, said array comprising at least two different positionally distinct target template nucleic acids;
(b) contacting said nucleic acid array with at least one nucleotide and a template-dependent nucleic acid polymerase; and
(c) determining the incorporation of the nucleotide directed by one of the template nucleic acids in a primer hybridized to the target template nucleic acid.
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Abstract
Means for simultaneous parallel sequence analysis of a large number of biological polymer macromolecules. Apparatus and methods may use fluorescent labels in repetitive chemistry to determine terminal monomers on solid phase immobilized polymers. Reagents which specifically recognize terminal monomers are used to label polymers at defined positions on a solid substrate.
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Citations
11 Claims
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1. A method for determining the incorporation of nucleotides comprising:
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(a) providing a nucleic acid array, said array comprising at least two different positionally distinct target template nucleic acids;
(b) contacting said nucleic acid array with at least one nucleotide and a template-dependent nucleic acid polymerase; and
(c) determining the incorporation of the nucleotide directed by one of the template nucleic acids in a primer hybridized to the target template nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification