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Sequencing of surface immobilized polymers utilizing microflourescence detection

  • US 20060172327A1
  • Filed: 01/05/2006
  • Published: 08/03/2006
  • Est. Priority Date: 12/06/1990
  • Status: Active Grant
First Claim
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1. A method for determining the incorporation of nucleotides comprising:

  • (a) providing a nucleic acid array, said array comprising at least two different positionally distinct target template nucleic acids;

    (b) contacting said nucleic acid array with at least one nucleotide and a template-dependent nucleic acid polymerase; and

    (c) determining the incorporation of the nucleotide directed by one of the template nucleic acids in a primer hybridized to the target template nucleic acid.

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