Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
First Claim
1. A process for the production of polyunsaturated fatty acids in an organism, which comprises the following steps:
- (a) introducing, into the organism, at least one nucleic acid sequence with the sequence shown in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18 or SEQ ID NO;
20, which codes for a polypeptide with lysophosphatidic acid acyltransferase activity;
or (b) introducing, into the organism, at least one nucleic acid sequence with the sequence shown in SEQ ID NO;
22, SEQ ID NO;
24 or SEQ ID NO;
26, which codes for a polypeptide with glycerol-3-phosphate acyltransferase activity;
or (c) introducing, into the organism, at least one nucleic acid sequence with the sequence shown in SEQ ID NO;
28, SEQ ID NO;
30 or SEQ ID NO;
32 which codes for a polypeptide with diacylglycerol acyltransferase activity;
or (d) introducing, into the organism, at least one nucleic acid sequence with the sequence shown in SEQ ID NO;
34 or SEQ ID NO;
36, which codes for a polypeptide with lecithin cholesterol acyltransferase activity;
or (e) introducing, into the organism, at least one nucleic acid sequence which can be derived from the coding sequence in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
1, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, SEQ ID NO;
34 or SEQ ID NO;
36 as the result of the degeneracy of the genetic code, or (f) introducing, into the organism, at least one derivative of the nucleic acid sequence shown in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
1, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, SEQ ID NO;
34 or SEQ ID NO;
36, which code for polypeptides with the amino acid sequence shown in SEQ ID NO;
2, SEQ ID NO;
5, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
15, SEQ ID NO;
17, SEQ ID NO;
19, SEQ ID NO;
21, SEQ ID NO;
23, SEQ ID NO;
25, SEQ ID NO;
27, SEQ ID NO;
29, SEQ ID NO;
31, SEQ ID NO;
33, SEQ ID NO;
35 or SEQ ID NO;
37 and which have at least 40% homology at the amino acid level with SEQ ID NO;
2, SEQ ID NO;
5, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
15, SEQ ID NO;
17, SEQ ID NO;
19, SEQ ID NO;
21, SEQ ID NO;
23, SEQ ID NO;
25, SEQ ID NO;
27, SEQ ID NO;
29, SEQ ID NO;
31, SEQ ID NO;
33, SEQ ID NO;
35 or SEQ ID NO;
37 and have an equivalent lysophosphatidic acid acyltransferase activity, glycerol-3-phosphate acyltransferase activity, diacylglycerol acyltransferase activity or lecithin cholesterol acyltransferase activity, and (g) culturing and harvesting the organism.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which code for polypeptides with acyl transferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences which code for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids. The invention furthermore relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences according to the invention, vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.
-
Citations
30 Claims
-
1. A process for the production of polyunsaturated fatty acids in an organism, which comprises the following steps:
-
(a) introducing, into the organism, at least one nucleic acid sequence with the sequence shown in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18 or SEQ ID NO;
20, which codes for a polypeptide with lysophosphatidic acid acyltransferase activity;
or(b) introducing, into the organism, at least one nucleic acid sequence with the sequence shown in SEQ ID NO;
22, SEQ ID NO;
24 or SEQ ID NO;
26, which codes for a polypeptide with glycerol-3-phosphate acyltransferase activity;
or(c) introducing, into the organism, at least one nucleic acid sequence with the sequence shown in SEQ ID NO;
28, SEQ ID NO;
30 or SEQ ID NO;
32 which codes for a polypeptide with diacylglycerol acyltransferase activity;
or(d) introducing, into the organism, at least one nucleic acid sequence with the sequence shown in SEQ ID NO;
34 or SEQ ID NO;
36, which codes for a polypeptide with lecithin cholesterol acyltransferase activity;
or(e) introducing, into the organism, at least one nucleic acid sequence which can be derived from the coding sequence in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
1, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, SEQ ID NO;
34 or SEQ ID NO;
36 as the result of the degeneracy of the genetic code, or(f) introducing, into the organism, at least one derivative of the nucleic acid sequence shown in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
1, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18, SEQ ID NO;
20, SEQ ID NO;
22, SEQ ID NO;
24, SEQ ID NO;
26, SEQ ID NO;
28, SEQ ID NO;
30, SEQ ID NO;
32, SEQ ID NO;
34 or SEQ ID NO;
36, which code for polypeptides with the amino acid sequence shown in SEQ ID NO;
2, SEQ ID NO;
5, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
15, SEQ ID NO;
17, SEQ ID NO;
19, SEQ ID NO;
21, SEQ ID NO;
23, SEQ ID NO;
25, SEQ ID NO;
27, SEQ ID NO;
29, SEQ ID NO;
31, SEQ ID NO;
33, SEQ ID NO;
35 or SEQ ID NO;
37 and which have at least 40% homology at the amino acid level with SEQ ID NO;
2, SEQ ID NO;
5, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
15, SEQ ID NO;
17, SEQ ID NO;
19, SEQ ID NO;
21, SEQ ID NO;
23, SEQ ID NO;
25, SEQ ID NO;
27, SEQ ID NO;
29, SEQ ID NO;
31, SEQ ID NO;
33, SEQ ID NO;
35 or SEQ ID NO;
37 and have an equivalent lysophosphatidic acid acyltransferase activity, glycerol-3-phosphate acyltransferase activity, diacylglycerol acyltransferase activity or lecithin cholesterol acyltransferase activity, and(g) culturing and harvesting the organism. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 24, 25, 29, 30)
-
-
11. An isolated nucleic acid sequence selected from the group consisting of:
-
(a) a nucleic acid sequence with the sequence shown in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18 or SEQ ID NO;
20,(b) nucleic acid sequences which, as the result of the degeneracy of the genetic code, can be derived from the coding sequence in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
1, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18 or SEQ ID NO;
20,(c) derivatives of the nucleic acid sequence shown in SEQ ID NO;
1, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
9, SEQ ID NO;
11, SEQ ID NO;
13, SEQ ID NO;
14, SEQ ID NO;
16, SEQ ID NO;
18 or SEQ ID NO;
20, which code for polypeptides with the amino acid sequence shown in SEQ ID NO;
2, SEQ ID NO;
5, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
15, SEQ ID NO;
17, SEQ ID NO;
19 or SEQ ID NO;
21 and which have at least 40% homology at the amino acid level with SEQ ID NO;
2, SEQ ID NO;
5, SEQ ID NO;
8, SEQ ID NO;
10, SEQ ID NO;
12, SEQ ID NO;
15, SEQ ID NO;
17, SEQ ID NO;
19 or SEQ ID NO;
21 and have lysophosphatidic acid acyltransferase activity. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 27, 28)
-
-
12. An isolated nucleic acid sequence selected from the group consisting of:
-
(a) a nucleic acid sequence with the sequence shown in SEQ ID NO;
22, SEQ ID NO;
24 or SEQ ID NO;
26,(b) nucleic acid sequences which, as the result of the degeneracy of the genetic code, can be derived from the coding sequence in SEQ ID NO;
22, SEQ ID NO;
24 or SEQ ID NO;
26,(c) derivatives of the nucleic acid sequence shown in SEQ ID NO;
22, SEQ ID NO;
24 or SEQ ID NO;
26, which code for polypeptides with the amino acid sequence shown in SEQ ID NO;
23, SEQ ID NO;
25 or SEQ ID NO;
27 and have at least 40% homology at the amino acid level with SEQ ID NO;
23, SEQ ID NO;
25 or SEQ ID NO;
27 and have glycerol-3-phosphate acyltransferase activity.
-
-
13. An isolated nucleic acid sequence selected from the group consisting of:
-
(a) a nucleic acid sequence with the sequence shown in SEQ ID NO;
28, SEQ ID NO;
30 or SEQ ID NO;
32,(b) nucleic acid sequences which, as the result of the degeneracy of the genetic code, can be derived from the coding sequence in SEQ ID NO;
28, SEQ ID NO;
30 or SEQ ID NO;
32,(c) derivatives of the nucleic acid sequence shown in SEQ ID NO;
28, SEQ ID NO;
30 or SEQ ID NO;
32, which code for polypeptides with the amino acid sequence shown in SEQ ID NO;
29, SEQ ID NO;
31 or SEQ ID NO;
33 and have at least 40% homology at the amino acid level with SEQ ID NO;
29, SEQ ID NO;
31 or SEQ ID NO;
33 and which have diacylglycerol acyltransferase activity.
-
-
14. An isolated nucleic acid sequence selected from the group consisting of:
-
(a) a nucleic acid sequence with the sequence shown in SEQ ID NO;
34 or SEQ ID NO;
36,(b) nucleic acid sequences which, as the result of the degeneracy of the genetic code, can be derived from the coding sequence in SEQ ID NO;
34 or SEQ ID NO;
36,(c) derivatives of the nucleic acid sequence shown in SEQ ID NO;
34 or SEQ ID NO;
36, which code for polypeptides with the amino acid sequence shown in SEQ ID NO;
35 or SEQ ID NO;
37 and which have at least 40% homology at the amino acid level with SEQ ID NO;
35 or SEQ ID NO;
37 and have lecithin cholesterol acyltransferase activity.
-
-
26. (canceled)
Specification