Kidney derived stem cells and methods for their isolation, differentiation and use
First Claim
Patent Images
1. An isolated or purified mammalian multipotent renal progenitor cell (MRPC) that is antigen positive for vimentin and Oct-4, and is antigen negative for zona occludens, cytokeratin, and major histocompatibility Class I and II molecules.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention relates generally to methods for isolation and culture of kidney stem cells, cells isolated by the methods, and therapeutic uses for those cells.
-
Citations
71 Claims
- 1. An isolated or purified mammalian multipotent renal progenitor cell (MRPC) that is antigen positive for vimentin and Oct-4, and is antigen negative for zona occludens, cytokeratin, and major histocompatibility Class I and II molecules.
-
30. A method for isolating a multipotent renal progenitor cell (MRPC), comprising:
(a) culturing renal cells in an aqueous medium consisting essentially of DMEM-LG, MCDB-201, insulin-transferrin-selenium (ITS), dexamethasone, ascorbic acid 2-phosphate, penicillin, streptomycin and fetal calf serim (FCS) and platelet derived growth factor (PDGF-BB), epidermal growth factor (EGF), and leukemia inhibitory factor (LIF) for about four weeks. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 61, 62, 63, 64, 65, 66, 67, 68, 69)
-
54. A method of repairing damaged tissue in a subject in need of such repair comprising administering exogenous molecules to a subject so that endogenous MRPCs are stimulated to proliferate and differentiate into different cell lineages of the kidney.
-
56. A method of using MRPCs to identify genetic polymorphisms associated with physiologic abnormalities, comprising
(a) isolating the MRPCs from a statistically significant population of individuals from whom phenotypic data can be obtained, (b) expanding the MRPCs from the statistically significant population of individuals in culture to establish MRPC cultures, (c) identifying at least one genetic polymorphism in the cultured MRPCs, (d) inducing the cultured MRPCs to differentiate, and (e) characterizing aberrant metabolic processes associated with the at least one genetic polymorphism by comparing the differentiation pattern exhibited by an MRPC having a normal genotype with the differentiation pattern exhibited by an MRPC having an identified genetic polymorphism.
-
58. A method of using MRPCs to characterize cellular responses to biologic or pharmacologic agents comprising
(a) culture expanding the MRPCs isolated from a statistically significant population of individuals so as to establish a plurality of MRPC cultures, (b) contacting the MRPC cultures with one or more biologic or pharmacologic agents, (c) identifying one or more cellular responses to the one or more biologic or pharmacologic agents, and (d) comparing the one or more cellular responses of the MRPC cultures from individuals in the statistically significant population.
Specification