Common marmoset embryonic stem cell lines
First Claim
1. A purified preparation of embryonic stem cells derived from common marmoset (Callithrix jacchus), which (i) is capable of prolonged undifferentiated proliferation in vitro, (ii) maintains, during prolonged culture, a karyotype in which all the chromosomal characteristics of common marmoset are present without noticeable alteration;
- (iii) is capable of differentiation into all three embryonic germ layers (ectoderm, endoderm and mesoderm) even after prolonged culture; and
(iv) is capable of teratoma formation in vivo.
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Accused Products
Abstract
The present invention relates to novel common marmoset (Callithrix jacchus) embryonic stem cell cultures and cell lines, and their use. The common marmoset embryonic cells of the present invention (i) are capable of prolonged undifferentiated proliferation in vitro, (ii) maintain, during prolonged culture, a karyotype in which all the chromosomal characteristics of common marmoset are present without noticeable alteration; (iii) are capable of differentiation into all three embryonic germ layers (ectoderm, endoderm and mesoderm) even after prolonged culture; and (iv) are capable of teratoma formation in vivo. The stem cells preferably are totipotent.
1 Citation
30 Claims
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1. A purified preparation of embryonic stem cells derived from common marmoset (Callithrix jacchus), which (i) is capable of prolonged undifferentiated proliferation in vitro, (ii) maintains, during prolonged culture, a karyotype in which all the chromosomal characteristics of common marmoset are present without noticeable alteration;
- (iii) is capable of differentiation into all three embryonic germ layers (ectoderm, endoderm and mesoderm) even after prolonged culture; and
(iv) is capable of teratoma formation in vivo. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 15, 16, 17)
- (iii) is capable of differentiation into all three embryonic germ layers (ectoderm, endoderm and mesoderm) even after prolonged culture; and
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12. A cell line developed from embryonic stem cells of common marmoset (Callithrix jacchus), which (i) is capable of prolonged undifferentiated proliferation in vitro, (ii) maintains, during prolonged culture, a karyotype in which all the chromosomal characteristics of common marmoset are present without noticeable alteration;
- and (iii) is capable of differentiation into all three embryonic germ layers (ectoderm, endoderm and mesoderm) even after prolonged culture, and (iv) is capable of teratoma formation in vivo.
- View Dependent Claims (13, 14)
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18. A common marmoset (Callithrix jacchus) carrying a genetic modification resulting from the introduction into said common marmoset genetically modified common marmoset embryonic stem cells which (i) are capable of prolonged undifferentiated proliferation in vitro, (ii) maintain, during prolonged culture, a karyotype in which all the chromosomal characteristics of common marmoset are present without noticeable alteration;
- (iii) are capable of differentiation into all three embryonic germ layers (ectoderm, endoderm and mesoderm) even after prolonged culture; and
(iv) are capable of teratoma formation in vivo. - View Dependent Claims (19)
- (iii) are capable of differentiation into all three embryonic germ layers (ectoderm, endoderm and mesoderm) even after prolonged culture; and
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20. An immunodeficient rodent having embryonic stem cells of common marmoset (Callithrix jacchus) transplanted therein, wherein said cells (i) are capable of prolonged undifferentiated proliferation in vitro, (ii) maintain, during prolonged culture, a karyotype in which all the chromosomal characteristics of common marmoset are present without noticeable alteration;
- (iii) are capable of differentiation into all three embryonic germ layers (ectoderm, endoderm and mesoderm) even after prolonged culture, and (iv) are capable of teratoma formation in vivo.
- View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
Specification