Method and apparatus for detecting biomolecular interactions

• US 20060181704A1
• Filed: 04/13/2006
• Published: 08/17/2006
• Est. Priority Date: 10/30/2000
• Status: Active Grant

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Li, Peter Y., Cunningham, Brian T., DeFilippi, Frank
• Granted Patent: US 7,148,964 B2
• Time in Patent Office: Days
• US Class Current: 356/326
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 2201/0635 Structured illumination, e.g. with grating
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides
• CPC Class Codes: G01N 2201/0833 Fibre array at detector, resolving
• CPC Class Codes: G01N 2201/0846 Fibre interface with sample, e.g. for spatial resolution
• CPC Class Codes: G01N 2201/1248 Validating from signal shape, slope, peak
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 21/06 Means for illuminating specimens
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: G02B 5/1814 structurally combined with one or more further optical elements, e.g. lenses, mirrors, prisms or other diffraction gratings G02B5/189 takes precedence

Abstract

Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.

Claims

1-9. -9. (canceled)

10. A biosensor imaging system comprising:

a light source generating white light;

a collimator for collimating said white light said collimated light being directed onto a surface of a biosensor;

an imaging spectrometer receiving reflected light from said biosensor, and

software calculating a peak wavelength value (PWV) of said reflected light.

11. The invention of claim 10 wherein said light source is a white light source.

12. The invention of claim 10 wherein said biosensor is arranged as an array of rows and columns of discrete detection areas.

13. The invention of claim 12 wherein said biosensor is coupled to a liquid containing vessel.

14. The invention of claim 10 wherein said imaging system further comprises an illuminating optical fiber coupled to the light source and wherein said illuminating optical fiber illuminates a bottom surface of said biosensor.

15. The invention of claim 10 further comprising an optical fiber for illuminating said biosensor.

16. The invention of claim 10 wherein said software enables a visual representation of said calculated PWV of said reflected light.

17-33. -33. (canceled)