Primer for nucleic acid detection
First Claim
Patent Images
1. An isolated nucleic acid molecule comprising:
- a 5′
end;
a 3′
end; and
a label on a nucleotide, wherein the labeled nucleotide is flanked by two nucleotides whose complement nucleotides change a detectable signal from the label when said isolated nucleic acid molecule is hybridized with its complementary nucleic acid molecule.
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Abstract
This application provides universal labeled primers for detection and amplification of nucleic acid molecules. These universal primers can be attached to the 5′-end of a target sequence-specific primer. In particular examples, the universal primer includes a labeled nucleotide flanked on both sides a nucleotide whose complement nucleotides changes a detectable signal from the label when the universal primer hybridizes with its complementary nucleic acid molecule. Also disclosed are methods of using the universal primer in nucleic acid amplification, such as real-time PCR.
30 Citations
49 Claims
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1. An isolated nucleic acid molecule comprising:
-
a 5′
end;
a 3′
end; and
a label on a nucleotide, wherein the labeled nucleotide is flanked by two nucleotides whose complement nucleotides change a detectable signal from the label when said isolated nucleic acid molecule is hybridized with its complementary nucleic acid molecule. - View Dependent Claims (2, 3, 6, 7, 8, 9, 10, 17, 18, 29, 30, 31, 32, 33, 34, 35, 36, 38, 47, 48)
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4-5. -5. (canceled)
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11-16. -16. (canceled)
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19. An isolated nucleic acid molecule comprising 5′
- -CTCSXXX(n)-3′
(SEQ ID NO;
22), wherein a fluorophore is attached to “
T” and
n comprises 0-11 nucleotides.
- -CTCSXXX(n)-3′
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20-28. -28. (canceled)
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37. (canceled)
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39-46. -46. (canceled)
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49. (canceled)
Specification