Microfluidic chip for multiple bioassay and method of manufacturing the same

• US 20060188906A1
• Filed: 01/20/2006
• Published: 08/24/2006
• Est. Priority Date: 01/20/2005
• Status: Active Grant

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Min, Jun-hong, Kim, Joon-ho, Lee, Soo-suk, Park, Hyun-gyu, Park, Kyung-hwan
• Granted Patent: US 7,670,772 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 2219/00522 in a multiple parallel arrangement
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00632 Introduction of reactive groups to the surface
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00657 One-dimensional arrays
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites

Abstract

Provided are a microfluidic chip for multiple bioassay and a method of manufacturing the same. The method includes: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate; forming probe immobilization regions at intersections between the probe channels and the sample channels; and forming blocking walls in the probe channels before and after each of the probe immobilization regions to prevent mixing of target sample. Since the probes are immobilized in the microfluidic channels after the formation of the microfluidic channels, difficulty in connecting fluidic channels after the immobilization of probes on a substrate can be removed. In addition, a microfluidic platform for a multiple bioassay into which a plurality of samples can be simultaneously loaded can be formed.

Claims

1. A method of forming a microfluidic chip for a multiple bioassay, the method comprising:

forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate;

forming probe immobilization regions at intersections between the probe channels and the sample channels; and

forming blocking walls in the probe channels before and after each of the probe immobilization regions to prevent mixing of target samples.

2. The method of claim 1, wherein the probes are biomolecules selected from the group consisting of DNA, RNA, peptide nucleic acid (PNA), locked nucleic acid (LNA), peptide, and protein.

3. The method of claim 1, wherein the probe immobilization regions are formed by photopolymerizing a polymer having biomolecule binding groups.

4. The method of claim 3, wherein the polymer is glycidyl methacrylate (GMA) polymer.

5. The method of claim 1, wherein the blocking walls are formed by photopolymerizing a polymer that does not have a biomolecule binding group.

6. The method of claim 5, wherein the polymer is polyethyleneglycol diacrylate (PEG-DA) polymer.

7. The method of claim 1, wherein the channel structure is a single layer composed of glass, PDMS, or polymer.

8. The method of claim 1, wherein the substrate is composed of silicon or glass.

9. The method of claim 1, wherein the height of the probe immobilization regions does not reach the ceiling of the microfluidic channels.

10. The method of claim 9, wherein, in the forming of the probe immobilization regions, a polymer is photopolymerized while a pressure is applied to the channel structure, and the pressure is removed after the completion of the photopolymerization.

11. The method of claim 9, wherein, in the forming of the probe immobilization regions, a polymer is partially photopolymerized by UV dose control.

12. A microfluidic chip for a multiple bioassay, the microfluidic chip comprising:

a substrate;

a singe-layered channel structure coupled to the substrate;

a plurality of sample channels and a plurality of probe channels formed in the channel structure;

probe immobilization regions formed at intersections between the sample channel and the probe channels; and

blocking walls formed in the probe channels before and after each of the probe immobilization regions to prevent mixing of target samples.