Polarization detection
First Claim
1. An optical device for receiving light, the device comprising:
- a polarizing beam splitter (PBS) that substantially reflects light of a first polarization and substantially transmits light of a second polarization orthogonal to the first polarization;
a reflector positioned to reflect light transmitted by the PBS towards the PBS; and
a detector positioned to detect light reflected by the PBS and/or light reflected by the reflector.
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Accused Products
Abstract
Disclosed is an optical device for receiving light having a wavelength between 400 nm and 680 nm. The device has a polarizing beam splitter (PBS) that comprises a substrate having a first and second surface, at least one of which being coated with a substantially parallel array of elongated conducting elements, wherein the PBS reflects light of a first polarity and transmits light of the opposite polarity; and a reflector positioned to reflect the opposite polarity light back through the PBS to the front surface, wherein the reflector does not substantially alter the polarity of light at any position. The device can be used to detect fluorescence polarization of a sample.
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Citations
44 Claims
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1. An optical device for receiving light, the device comprising:
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a polarizing beam splitter (PBS) that substantially reflects light of a first polarization and substantially transmits light of a second polarization orthogonal to the first polarization;
a reflector positioned to reflect light transmitted by the PBS towards the PBS; and
a detector positioned to detect light reflected by the PBS and/or light reflected by the reflector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 23, 24, 43)
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18-22. -22. (canceled)
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25-27. -27. (canceled)
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28. A polarizing beam splitter (PBS) comprising:
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an optically transparent substrate, having a front surface and a rear surface angled relative to the front surface;
a generally parallel array of elongated elements disposed on the front surface of the substrate configured to substantially reflect light of a first polarization, and substantially transmit light of a second polarization orthogonal to the first polarization; and
a reflective coating disposed on the rear surface. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36)
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37. A method of evaluating fluorescence polarization, the method comprising:
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collecting light from a plurality of distinguishable positions on an illuminated object;
separating the collected light according to polarity using an optical element that reflects light polarized in a first plane and transmits light in a second plane, orthogonal to the first plane;
projecting the reflected light and the transmitted light onto a detector surface; and
comparing the reflected and the transmitted light for each of the distinguishable positions to thereby determine fluorescence polarization at each of the distinguishable positions. - View Dependent Claims (38)
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39. A method comprising:
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providing a plurality of spatially distinct nucleic acid samples and amplification reagents that comprises a fluorophore attached to a nucleic acid primer;
concurrently amplifying each sample of the plurality; and
during the amplifying, concurrently detecting fluorescence polarization information associated with the fluorophore from each sample of the plurality, wherein the detecting comprises separating first and second polarity light using an element that reflects first polarity light and transmits second polarity light, wherein the first polarity light is polarized in a first plane and the second polarity light is polarized in a plane orthogonal to the first plane. - View Dependent Claims (40, 41, 42)
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44-49. -49. (canceled)
Specification