Method of providing readily available cellular material derived from peripheral blood, and a composition thereof
First Claim
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1. Peripheral blood stem cells from a mammal, wherein said peripheral blood stem cells are in a number per volume that is at least 7 times greater than naturally-occurring peripheral blood;
- and wherein the peripheral blood stem cells have a three-dimensional geometry and cell-to-cell support and cell-to-cell geometry that is essentially the same as stem cells of naturally-occurring peripheral blood.
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Abstract
The present invention is directed to the TVEMF-expansion of mammalian peripheral blood stem cells, preferably CD34+/CD38− cells, to compositions resulting from the TVEMF-expanded cells, and to a method of treating disease or repairing tissue with the compositions.
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Citations
55 Claims
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1. Peripheral blood stem cells from a mammal,
wherein said peripheral blood stem cells are in a number per volume that is at least 7 times greater than naturally-occurring peripheral blood; - and
wherein the peripheral blood stem cells have a three-dimensional geometry and cell-to-cell support and cell-to-cell geometry that is essentially the same as stem cells of naturally-occurring peripheral blood. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 42, 48)
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- 11. Peripheral blood stem cells from a mammal, wherein said peripheral blood stem cells are TVEMF-expanded.
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22. A process for preparing a peripheral blood stem cell composition comprising the steps of:
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a. placing a peripheral blood mixture in a culture chamber of a TVEMF-bioreactor; and
b. subjecting the peripheral blood mixture to a TVEMF and TVEMF-expanding the peripheral blood stem cells in the TVEMF-bioreactor to prepare the peripheral blood stem cell composition. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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- 53. A method of researching a disease state comprising introducing a TVEMF-expanded stem cell into a test system for the disease state.
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55. Bone marrow stem cells from a mammal, wherein said bone marrow stem cells are TVEMF-expanded.
Specification