Cancer markers and detection methods
First Claim
1. A cancer detection reagent set comprising cancer detection reagents corresponding to at least 10 general cancer markers.
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Abstract
The present invention relates to cancer markers and methods of detecting cancer markers in a sample. The sample may be peripheral blood. Cancer markers are most commonly mutated or abnormal DNA sequences associated with metastatic cancer. Markers may be detected using PCR, microarrays, or other nucleic acid or peptide-based assays. These methods may be used for a variety of diagnostic purposes, including initial, early-stage or later diagnosis of cancer, particularly metastatic cancer and monitoring of cancer or treatment progression. The cancer markers may also be used to create a cancer marker profile. Treatment may be directed based on this profile. Additionally, methods using blood may provide a cancer marker profile of mutations or abnormalities found in at least one of several tumors in the body, instead of merely one tumor. The invention also include kits, such as primer kits, and microarrays for use in performing the various methods.
61 Citations
24 Claims
- 1. A cancer detection reagent set comprising cancer detection reagents corresponding to at least 10 general cancer markers.
- 4. A cancer detection reagent set comprising cancer detection reagents corresponding to at least 10 colon cancer markers.
- 7. A cancer detection reagent set comprising cancer detection reagents corresponding to at least 10 lung cancer markers.
- 10. A cancer detection reagent set comprising cancer detection reagents corresponding to at least 10 lymph cancer markers.
- 13. A cancer detection reagent set comprising cancer detection reagents corresponding to at least 10 breast cancer markers.
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21. A method of detecting a cancer marker in a sample comprising:
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extracting mRNA from the sample;
creating cDNA from the mRNA;
performing at least 10 separate PCR reduction reactions using the cDNA as a template and at least 10 different single primers, with a different single primer in each separate PCR reduction reaction; and
analyzing the product of each of the 10 separate PCR reduction reactions to determine the presence or absence of primer-amplified DNA molecules, where presence of primer-amplified DNA molecules in any PCR reduction reaction indicates the presence of a cancer marker;
wherein the 10 different single primers each have an antisense sequence corresponding to a different cancer marker. - View Dependent Claims (22)
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23. A method of detecting cancer markers in a sample comprising:
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isolating sample nucleic acids from the sample;
placing the sample nucleic acids on a microarray having DNA molecules operable to detect and distinguish at least 10 corresponding cancer markers under conditions sufficient to allow detectable and specific binding of sample nucleic acids to complementary DNA molecules of the microarray; and
detecting binding of sample nucleic acids to the microarray, wherein binding of sample nucleic acids to a DNA molecule on the microarray indicates the presence of a cancer marker corresponding to that DNA molecule in the sample. - View Dependent Claims (24)
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Specification