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Affinity based system for detecting particulates in a fluid

  • US 20060194264A1
  • Filed: 10/11/2005
  • Published: 08/31/2006
  • Est. Priority Date: 12/20/1996
  • Status: Abandoned Application
First Claim
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1. An apparatus for detecting a particulate in a fluid, the apparatus comprising a platform comprising a substrate having a surface, wherein said surface defines a detection chamber comprising an area that is coated with a specific binding reagent that specifically binds to the particulate to be detected;

  • a fluid sample input means in fluid communication with the detection chamber that is coated with the specific binding reagent;

    a wash buffer reservoir containing a wash buffer in fluid communication with the detection chamber that is coated with the specific binding reagent;

    a fluid waste receptacle in fluid communication with the detection chamber that is coated with the specific binding reagent;

    wherein an amount of a fluid sample comprising a particulate is moved from the fluid sample input means to the detection chamber and incubated thereon for a time sufficient to result in specific binding between the particulate in the fluid sample and the specific binding reagent in the detection chamber; and

    wherein the fluid sample is replaced with the wash buffer and displaced into the fluid waste receptacle; and

    wherein the wash buffer is further displaced into the fluid waste receptacle; and

    wherein a particulate specifically bound to the detection chamber is specifically bound to the specific binding reagent and is detected thereupon.

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