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Polymorphic DNA fragments and uses thereof

  • US 20060199198A1
  • Filed: 01/13/2006
  • Published: 09/07/2006
  • Est. Priority Date: 02/22/1999
  • Status: Abandoned Application
First Claim
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1. A method of making a reference library comprising a mixture of heterogeneous nucleic acid fragments, comprising:

  • digesting pooled nucleic acid from a plurality of individuals, comprising first restriction sites s, with a first restriction endonuclease S, to produce a mixture of restriction fragments;

    forming a first population of single stranded nucleic DNA fragments from a first subpopulation of said restriction fragments, wherein said first subpopulation of restriction fragments comprises a second restriction site t which is different from said first restriction site;

    forming a second population of single stranded DNA fragments from a second subpopulation of said restriction fragments, wherein said second subpopulation of said restriction fragments do not contain said second restriction site, and wherein said first single stranded DNA fragments are complementary to said second single stranded DNA fragments when said single stranded DNA fragments are derived from the same restriction fragment;

    hybridizing the first and second populations of single stranded DNA fragments to form a population of duplexes; and

    isolating said duplexes to form a reference population of restriction fragments.

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