Self-assembly of molecules using combinatorial hybridization
First Claim
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1. An array comprising a plurality of conjugates and a plurality of anchors, wherein:
- each of the conjugates comprises a molecule bound to a nucleic acid fragment, wherein the molecule of each conjugate is not the same as the molecule of any of the other conjugates;
each of the anchors is immobilized on a surface and comprises at least two nucleic acid fragments; and
the nucleic acid fragment of each conjugate is hybridized to at least one of the nucleic acid fragments of the anchors.
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Abstract
Simple and convenient methods for arranging molecules of interest in a pre-determined pattern are described. The methods use combinatorial hybridization based on interactions between complementary nucleic acid sequences to arrange the molecules of interest. The resulting arrangements, kits containing the components used in the methods, and methods of using the resulting arrangements are also disclosed.
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Citations
42 Claims
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1. An array comprising a plurality of conjugates and a plurality of anchors, wherein:
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each of the conjugates comprises a molecule bound to a nucleic acid fragment, wherein the molecule of each conjugate is not the same as the molecule of any of the other conjugates;
each of the anchors is immobilized on a surface and comprises at least two nucleic acid fragments; and
the nucleic acid fragment of each conjugate is hybridized to at least one of the nucleic acid fragments of the anchors. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of arranging molecules comprising:
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(a) immobilizing a first set of nucleic acid fragments with known sequences in a predetermined pattern on a surface to form anchors;
(b) contacting the anchors with a mixture comprising conjugates of a second set of nucleic acid fragments and the molecules, wherein the nucleic acid fragment in each conjugate has a sequence complementary to at least part of one of the nucleic acid fragments in the anchors; and
(c) incubating the anchors and the mixture for a time and under conditions sufficient for the conjugates to bind to the anchors, thereby arranging the molecules. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method of characterizing a protein comprising:
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(a) immobilizing a first set of nucleic acid fragments with known sequences in a predetermined pattern on a surface to form anchors;
(b) contacting the anchors with a mixture comprising conjugates of a second set of nucleic acid fragments and peptide fragments, wherein the nucleic acid fragment in each conjugate has a sequence complementary to at least part of one of the nucleic acid fragments in the anchors;
(c) incubating the anchors and the mixture for a time and under conditions sufficient for the conjugates to bind to the anchors to provide an array of anchor-conjugate complexes;
(d) contacting the array with the protein for a time and under conditions sufficient for the protein to bind to one or more of the complexes; and
(e) detecting the binding of the protein to the complexes to obtain a binding pattern;
wherein the binding pattern is characteristic of the protein. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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39. A kit for a protein assay comprising:
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a multi-well plate, each well containing anchors, each of which is immobilized on the surface of the well and comprises one or more of nucleic acid fragments; and
one or more peptide libraries, each library comprising conjugates, each of which comprising a nucleic acid fragment and a peptide, and wherein the nucleic acid fragment in each conjugate has a sequence complementary to at least part of one of the immobilized nucleic acid fragments in the anchors. - View Dependent Claims (40, 41, 42)
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Specification