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Incorporation of modified nucleotides by archaeon DNA polymerases and related methods

  • US 20060199214A1
  • Filed: 05/04/2006
  • Published: 09/07/2006
  • Est. Priority Date: 09/30/1999
  • Status: Abandoned Application
First Claim
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1. A method for site-specific incorporation of derivatized dideoxynucleotides into DNA comprising reacting an archaeon Family B DNA polymerase, a primed DNA template and nucleotide solution containing at least one derivatized dideoxynucleotide to produce fragments of DNA with the derivatized dideoxynucleoside covalently attached to the 3′

  • terminal residue wherein the derivatized dideoxynucleotide is incorporated more efficiently than the corresponding underivatized dideoxynucleotide.

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