Seeding implantable medical devices with cells
First Claim
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1. A method for seeding cells comprising:
- introducing cells into a lumen of an implantable medical device adapted to be implanted in a living body, wherein the lumen comprises a luminal surface comprising expanded polytetrafluoroethylene and wherein the lumen defines a longitudinal axis; and
applying centrifugation to the implantable medical device to rotate the implantable medical device around the axis.
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Abstract
The invention is directed to apparatus and methods for seeding an implantable medical device, such as a vascular prosthesis, with cells, such as endothelial cells. The invention supports techniques for seeding a luminal surface of the device with axial centrifugation. Cells are introduced in suspension into the lumen of the device, and the device is subjected to centrifugation around a longitudinal axis defined by the lumen. Axial centrifugation causes the cells to concentrate toward the luminal surface. Shortly after axial centrifugation, the seeded device can be presented for implantation in a patient.
74 Citations
32 Claims
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1. A method for seeding cells comprising:
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introducing cells into a lumen of an implantable medical device adapted to be implanted in a living body, wherein the lumen comprises a luminal surface comprising expanded polytetrafluoroethylene and wherein the lumen defines a longitudinal axis; and
applying centrifugation to the implantable medical device to rotate the implantable medical device around the axis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for seeding cells comprising:
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introducing cells into a lumen of an implantable medical device adapted to be implanted in a living body, wherein the lumen includes a luminal surface having recesses defined by nodes lifted from the surface, and wherein the lumen defines a longitudinal axis; and
applying centrifugation to the implantable medical device to rotate the implantable medical device around the axis. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23)
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24. An apparatus to apply centrifugation to an implantable medical device comprising:
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an adapter configured to mate with a rotor of a centrifuge proximate to an axis of rotation of the rotor, the adapter comprising a chamber that extends in the direction of the axis;
a tube configured to receive the implantable medical device, and further configured to be received in the chamber, the tube including an open end; and
a plug configured to seal the open end of the tube. - View Dependent Claims (25, 26, 27, 28, 29, 30)
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31. An apparatus to apply centrifugation to an implantable medical device comprising:
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an adapter means configured to mate with a rotor of a centrifuge proximate to an axis of rotation of the rotor, the adapter comprising a chamber; and
a container means configured to receive the implantable medical device, and further configured to be received in the chamber. - View Dependent Claims (32)
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Specification