Device and method for multiple analyte detection

• US 20060204401A1
• Filed: 05/10/2006
• Published: 09/14/2006
• Est. Priority Date: 04/03/1996
• Status: Active Grant

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Kowallis, Reid B., Albin, Michael, Winn-Deen, Emily S., Ragusa, Robert P., Raysberg, Yefim, Woudenberg, Timothy M.
• Granted Patent: US 8,067,226 B2
• Time in Patent Office: Days
• US Class Current: 422/81
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00599 Solution-phase processes
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/08 using multifield media
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2565/631 being a biochannel or pore
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 50/08 Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: Y10S 436/805 : Optical property
• CPC Class Codes: Y10S 436/807 : Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/808 : Automated or kit
• CPC Class Codes: Y10S 436/809 : Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/25 : including sample preparation

Abstract

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Claims

1. A device comprising:

a substrate;

a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the detection chambers and the inlet; and

detection reagents in the two or more detection chambers;

wherein the substrate comprises two or more layers and at least one of the two or more layers comprises a heat-conducting metal.

2. The device of claim 1, wherein the fluid communication comprises an individual channel for each detection chamber, for providing a connection between the inlet and each detection chamber.

3. The device of claim 1, wherein the heat-conducting metal comprises copper.

4. The device of claim 1, wherein the heat-conducting metal comprises aluminum.

5. The device of claim 1, further comprising an optically clear cover layer disposed over the sample distribution network.

6. The device of claim 1, wherein at least one of the two or more detection chambers comprises a dried detection reagent.

7. The device of claim 1, wherein the substrate defines at least two such sample-distribution networks.

8. The device of claim 1, further comprising means for modulating the temperature of the detection chambers, in the substrate.

9. The device of claim 1, wherein the two or more layers are laminated together.

10. A system comprising the device of claim 1 and an external temperature controller.

11. A device comprising:

a substrate;

a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) channel means providing fluid communication between the detection chambers and the inlet; and

detection reagents in the detection chambers;

wherein the substrate comprises two or more layers and a high thermal conductivity metal surface.

12. The device of claim 11, wherein the channel means comprises an individual channel for each detection chamber, for providing a fluid communication between the inlet and each detection chamber.

13. The device of claim 11, wherein the high thermal conductivity metal surface comprises copper.

14. The device of claim 11, wherein the high thermal conductivity metal surface comprises aluminum.

15. The device of claim 11, further comprising an optically clear cover layer disposed over the sample distribution network.

16. The device of claim 11, wherein at least one of the two or more detection chambers comprises a dried detection reagent.

17. The device of claim 11, wherein the substrate defines at least two such sample-distribution networks.

18. The device of claim 11, further comprising means for modulating the temperature of the detection chambers, in the substrate.

19. The device of claim 11, wherein the two or more layers are laminated together.

20. A system comprising the device of claim 11 and an external temperature controller.