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Nucleic acid affinity columns

  • US 20060204991A1
  • Filed: 02/22/2006
  • Published: 09/14/2006
  • Est. Priority Date: 03/11/1996
  • Status: Abandoned Application
First Claim
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1. A method of making a nucleic acid pool comprising a plurality of different nucleic acids, said method comprising the steps of:

  • a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 20 oligonucleotides having different predetermined nucleic acid sequences, and wherein;

    i) each different oligonucleotide is localized in a predetermined region of said surface;

    ii) the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2; and

    iii) said different oligonucleotides have an identical terminal 5

    nucleic acid sequence;

    b) amplifying said multiplicity of oligonucleotides at least about 10 fold to provide said nucleic acid pool.

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