Method for determining hair cycle markers
First Claim
1. An in vitro method for determining hair cycle phase in humans, comprising:
- a) providing a plurality of genetically encoded markers isolated from hair covered human skin or from human hair follicles which are differentially expressed at an anagenic phase of the hair cycle when compared to expression in cells in the catagenic phase of the hair cycle;
b) obtaining a sample of hair covered skin or human hair follicles;
c) analyzing the sample of b) for the presence and optionally the quantity of at least one genetically encoded molecule which is differentially expressed in anagenic and catagenic hair follicles and d) designating the sample as comprising healthy cells in the anagenic phase of the cycle if it contains markers which are expressed at higher levels in anagenic hair follicles or cells in regression in the catagenic phase if it contains molecules which are expressed at higher levels in catagenic hair follicles.
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Abstract
The invention relates to a method for determining hair cycle markers in vitro, test kits and biochips which are used to determine hair cycle markers and to the use of proteins, mRNA molecules, proteins or fragments thereof as hair cycle markers. The invention also relates to a test method which is used to detect the effectiveness of cosmetic and pharmaceutical active ingredients which influence the hair cycle, in addition to a screening method which is used to identify cosmetic or pharmaceutical active ingredients which influence the hair cycle and to a method for the production of a cosmetic and pharmaceutical preparation which influences the hair cycle.
5 Citations
22 Claims
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1. An in vitro method for determining hair cycle phase in humans, comprising:
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a) providing a plurality of genetically encoded markers isolated from hair covered human skin or from human hair follicles which are differentially expressed at an anagenic phase of the hair cycle when compared to expression in cells in the catagenic phase of the hair cycle;
b) obtaining a sample of hair covered skin or human hair follicles;
c) analyzing the sample of b) for the presence and optionally the quantity of at least one genetically encoded molecule which is differentially expressed in anagenic and catagenic hair follicles and d) designating the sample as comprising healthy cells in the anagenic phase of the cycle if it contains markers which are expressed at higher levels in anagenic hair follicles or cells in regression in the catagenic phase if it contains molecules which are expressed at higher levels in catagenic hair follicles. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16, 17, 18, 19, 20, 21, 22)
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14. A biochip for determining the hair cycle phase in human beings in vitro comprising a solid, i.e. rigid or flexible, carrier and a plurality of probes immobilized thereon which are capable of specifically binding to at least one molecule selected from the group consisting of SEQ ID NO:
- 1 to SEQ ID NO;
570 or the corresponding gene product.
- 1 to SEQ ID NO;
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15. A marker for determining hair cycle phase in human beings in vitro, selected from the group consisting of at least one molecule having a Swissprot Accession Number provided in column 8 of Table 8, a Swissprot Accession Number provided in column 9 of Table 7, a Swissprot Accession Number provided in column 9 of Table 9, a UniGene Accession Number provided in column 7 of Tables 2 to 6, and a Swissprot Accession Number in column 8 of Tables 2 to 6.
Specification