Infectious clones of RNA viruses and vaccines and diagnostic assays derived thereof
First Claim
1. A method of generating an infectious clone, said infectious clone based upon a positive strand RNA virus'"'"'s genome, said method comprising:
- producing a recombinant nucleic acid comprising at least one full-length DNA copy or at least one in vitro-transcribed RNA copy or a derivative of either said at least one DNA copy or said at least one in-vitro transcribed RNA copy, wherein the RNA virus'"'"'s genome is at least about 15 kb.
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Abstract
An infectious clone based on the genome of a wild-type RNA virus is produced by the process of providing a host cell not susceptible to infection by the wild-type RNA virus, providing a recombinant nucleic acid based on the genome of the wild-type RNA virus, transfecting the host cell with the recombinant nucleic acid and selecting for infectious clones. The recombinant nucleic acid comprises at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either. The infectious clones can be used in single or dual purpose vaccines and in viral vector vaccines.
125 Citations
24 Claims
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1. A method of generating an infectious clone, said infectious clone based upon a positive strand RNA virus'"'"'s genome, said method comprising:
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producing a recombinant nucleic acid comprising at least one full-length DNA copy or at least one in vitro-transcribed RNA copy or a derivative of either said at least one DNA copy or said at least one in-vitro transcribed RNA copy, wherein the RNA virus'"'"'s genome is at least about 15 kb. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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2. A method of generating an infectious clone based upon an RNA virus'"'"'s genome, said method comprising:
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producing a recombinant nucleic acid comprising at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either, and selecting infectious clones by transfecting a host cell with said recombinant nucleic acid wherein said host cell is not susceptible to infection to said virus. - View Dependent Claims (3, 4)
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5. A modified RNA virus comprising a recombinant nucleic acid, said recombinant nucleic acid comprising at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either.
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6. A vaccine comprising a modified RNA virus, said modified RNA virus comprising a recombinant nucleic acid which comprises at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either.
- 7. A cell culture infected with a modified RNA virus, said modified RNA virus comprising a recombinant nucleic acid which comprises at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either.
Specification