Electrodes linked via conductive oligomers to nucleic acids
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Accused Products
Abstract
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.
91 Citations
71 Claims
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1-59. -59. (canceled)
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60. A method of detecting a target nucleic acid sequence in a nucleic acid sample comprising
a) providing a solid support comprising an array of electrodes each comprising: -
i) a covalently attached anchor sequence; and
ii) a self-assembled monolayer;
b) contacting said array with at least one target sequence and a second nucleic acid sequence comprising a first region substantially complementary to said anchor sequence and a second region substantially complementary to a region of said target sequence, to form a hybridization complex;
wherein said hybridization complex further comprises an electron transfer moiety (ETM); and
c) detecting the presence of said ETM as an indication of the presence of said target nucleic acid sequence. - View Dependent Claims (61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71)
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Specification