Methods and compositions for assay readouts on multiple analytical platforms
First Claim
1. A method of identifying a segmented tag by size separation, the method comprising the steps of:
- providing a segmented tag comprising more than one subunits, each subunit having a position in the segmented tag and each being selected from a set of subunits consisting of a plurality of different nucleotides or oligonucleotides;
providing for each position of the segmented tag a fragment set, such fragment sets having successively larger nucleic acid fragments such that a shortest nucleic acid fragment of a next-larger fragment set has a length that is greater than or equal to that of a longest nucleic acid fragment of a next-smaller fragment set, and wherein each nucleic acid fragment within a fragment set has a different length and each fragment within a set has a one-to-one correspondence with a different subunit;
concatenating for each position of the segmented tag a nucleic acid fragment from its corresponding fragment set, each such nucleic acid fragment corresponding to the subunit at the position corresponding to its fragment set to form a concatenate; and
determining the length of the concatentate to identify the segmented tag.
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Abstract
The invention provides methods and compositions for reading out the results of multiplex assays on various analytical platforms, such as microarrays, bead arrays, electrophoresis devices, and the like. An important feature of the invention includes methods for converting different sets of oligonucleotide tags used for labeling into oligonucleotide tags specific for a particular analytical platform. The invention further includes compositions comprising oligonucleotide tags having convenient properties for labeling and conversion, particularly ligation tags that employ ligation reaction specificity as well as sequence specificity in order to discriminate between tags.
232 Citations
21 Claims
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1. A method of identifying a segmented tag by size separation, the method comprising the steps of:
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providing a segmented tag comprising more than one subunits, each subunit having a position in the segmented tag and each being selected from a set of subunits consisting of a plurality of different nucleotides or oligonucleotides;
providing for each position of the segmented tag a fragment set, such fragment sets having successively larger nucleic acid fragments such that a shortest nucleic acid fragment of a next-larger fragment set has a length that is greater than or equal to that of a longest nucleic acid fragment of a next-smaller fragment set, and wherein each nucleic acid fragment within a fragment set has a different length and each fragment within a set has a one-to-one correspondence with a different subunit;
concatenating for each position of the segmented tag a nucleic acid fragment from its corresponding fragment set, each such nucleic acid fragment corresponding to the subunit at the position corresponding to its fragment set to form a concatenate; and
determining the length of the concatentate to identify the segmented tag. - View Dependent Claims (3, 4, 5, 10, 11, 17)
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2. The method of claim 2 wherein said segmented tag is a sequence of nucleotides.
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6. A method of identifying members of a population of segmented tags, wherein each segmented tag of the population comprises a sequence of subunits selected from a plurality of different nucleotides or oligonucleotides, each subunit having a position within a segmented tag, the method comprising the steps of:
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(a) providing for each position of the segmented tags a fragment set, such fragment sets having successively larger nucleic acid fragments such that a shortest nucleic acid fragment of a next-larger fragment set has a length that is greater than or equal to that of a longest nucleic acid fragment of a next-smaller fragment set, and wherein each nucleic acid fragment within a fragment set has a different length and each fragment within a set has a one-to-one correspondence with a different subunit;
(b) concatenating for each position of each segmented tag nucleic acid fragments from the fragment set corresponding to each such position and corresponding to the subunit occupying such position to form for each segmented tag a concatenate; and
(c) separating the concatenates by length to identify the corresponding segmented tags. - View Dependent Claims (7, 8, 9)
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12. A set of ligation tags comprising a plurality of member oligonucleotides, each such member having a tag complement and each comprising:
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a length in the range of from six to twelve nucleotides;
a duplex stability with its tag complement equivalent to that of every other oligonucleotide member;
a first terminal nucleotide and a second terminal nucleotide selected so that whenever a member oligonucleotide forms a duplex with a tag complement of another member oligonucleotide, the first terminal nucleotide and the second nucleotide each form mismatches with respect to nucleotides of the tag complement with which they are paired.
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13. A method of identifying individual polynucleotides in a mixture, the method comprising the steps of:
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attaching to each individual polynucleotide in the mixture a different ligation tag to form tag-polynucleotide conjugates;
generating labeled ligation tags from the tag-polynucleotide conjugates; and
identifying the labeled ligation tags on a readout platform. - View Dependent Claims (14, 15)
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16. A method of generating a single stranded overhang in a cleavage of a double stranded DNA, the method comprising the steps of:
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providing a first recognition site of a nicking enzyme in a double stranded DNA, the nicking enzyme being capable of cleaving only a single strand of the double stranded DNA;
providing a second recognition site of a restriction endonuclease in the double stranded DNA, the restriction endonuclease being capable of cleaving both strands of the double stranded DNA, providing a cleavage segment in the double stranded DNA, the cleavage segment being disposed between and being immediately adjacent to the first recognition site and the second recognition site; and
cleaving the double stranded DNA with the nicking enzyme and the restriction endonuclease so that at a first end of the cleavage segment both strands of the double stranded DNA are cleaved and at a second end of the cleavage segment a single strand of the double stranded DNA is cleaved to produce a free cleavage segment oligonucleotide and a single stranded overhang.
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18. A composition of matter comprising a plurality of ligation tags selected from the group defined by the formulas:
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5′
-Y1—
N1N2-(Z)K-N3N4—
Y2where K is 1, 2, or 3;
Y. and Y2 are separately each A, C, G, or T;
N1, N2, N3, and N4 are separately each A, C, G, or T; and
Z is a dinucleotide, GT, TG, CA, or AC, with the proviso that whenever K is greater than one, each Z is separately GT, TG, CA, or AC.- View Dependent Claims (19, 20, 21)
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Specification