Capture and release assay system and method
First Claim
1. A method for detecting one or more component of interest in a fluid-borne sample in a microchannel of a microfluidic device, the method comprising the steps of:
- (i) flowing the fluid-borne sample comprising the one or more component of interest bound to a first labeled component binding moiety to form a first labeled complex through a binding channel region of the microchannel, which binding channel region comprises at least a second component-binding moiety that is reversibly bound to a wall surface of the binding channel region, thereby binding at least a portion of the one or more components of interest to the second component-binding moiety to form a second labeled complex;
(iii) releasing the second labeled complex comprising the first and second component binding moieties and the one or more component of interest from the binding channel region, thereby releasing the second labeled complex into the microchannel; and
, (iv) flowing the released second labeled complex through a detection channel region of the microchannel and detecting the second labeled complex.
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Abstract
A method for detecting one or more components of interest in a fluid-borne sample in a microchannel of a microfluidic device includes flowing the fluid-borne sample, in which the one or more components of interest are bound to a first labeled component binding moiety to form a first labeled complex, through a binding channel region of the microchannel. The binding channel region includes a second component-binding moiety reversibly bound to a wall surface of the binding channel region. At least a portion of the one or more components of interest is bound to the second component-binding moiety to form a second labeled complex. The second labeled complex is released from the binding channel region and flowed through a detection channel region of the microchannel, where the second labeled complex is detected.
55 Citations
21 Claims
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1. A method for detecting one or more component of interest in a fluid-borne sample in a microchannel of a microfluidic device, the method comprising the steps of:
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(i) flowing the fluid-borne sample comprising the one or more component of interest bound to a first labeled component binding moiety to form a first labeled complex through a binding channel region of the microchannel, which binding channel region comprises at least a second component-binding moiety that is reversibly bound to a wall surface of the binding channel region, thereby binding at least a portion of the one or more components of interest to the second component-binding moiety to form a second labeled complex;
(iii) releasing the second labeled complex comprising the first and second component binding moieties and the one or more component of interest from the binding channel region, thereby releasing the second labeled complex into the microchannel; and
,(iv) flowing the released second labeled complex through a detection channel region of the microchannel and detecting the second labeled complex. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification