Hyaluronan synthases and methods of making and using same
First Claim
1. A recombinant host cell comprising at least one expression construct comprising a hyaluronan synthase gene encoding a functionally active hyaluronan synthase such that the recombinant host cell is capable of producing hyaluronic acid, the functionally active hyaluronan synthase having an altered enzymatic activity as compared to a corresponding functionally active native hyaluronan synthase, and wherein the functionally active hyaluronan synthase has at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase, and wherein the at least one modified amino acid residue is a cysteine modified to an alanine or a serine residue.
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Abstract
A functionally active hyaluronan synthase having at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase such that the functionally active hyaluronan synthase has an altered enzymatic activity as compared to the corresponding functionally active native hyaluronan synthase is disclosed. Methods of producing hyaluronic acid utilizing a recombinant host cell having an expression construct encoding the functionally active hyaluronan synthase with altered enzymatic activity are also disclosed.
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Citations
45 Claims
- 1. A recombinant host cell comprising at least one expression construct comprising a hyaluronan synthase gene encoding a functionally active hyaluronan synthase such that the recombinant host cell is capable of producing hyaluronic acid, the functionally active hyaluronan synthase having an altered enzymatic activity as compared to a corresponding functionally active native hyaluronan synthase, and wherein the functionally active hyaluronan synthase has at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase, and wherein the at least one modified amino acid residue is a cysteine modified to an alanine or a serine residue.
- 17. A recombinant host cell comprising at least one expression construct comprising a hyaluronan synthase gene encoding a functionally active hyaluronan synthase such that the recombinant host cell is capable of producing hyaluronic acid, the functionally active hyaluronan synthase having at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase, and wherein the functionally active hyaluronan synthase has at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase, and wherein the at least one modified amino acid residue is a cysteine modified to an alanine or a serine residue.
- 33. A recombinant host cell comprising at least one expression construct comprising a hyaluronan synthase gene encoding a functionally active hyaluronan synthase such that the recombinant host cell is capable of producing hyaluronic acid, the functionally active hyaluronan synthase having an amino acid sequence comprising at least one of SEQ ID NOS:
Specification