Compositions and methods for neural cell production and stabilization
First Claim
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1. A method of stabilizing a neural cell comprising:
- (a) providing a pluripotent mammalian cell;
(b) culturing the pluripotent mammalian cell to produce a neural cell; and
(c) contacting the neural cell with a MEDII conditioned medium for greater than 2 passages to thereby stabilize the neural cell.
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Abstract
The present invention provides compositions and methods for mammalian neural cell production, their stabilization and their proliferation. More particularly, the present invention provides cellular differentiation methods employing culturing the cells on a cell line or in cell culture and further contacting the cells with MEDII conditioned medium for the generation of stable mammalian neural cells from pluripotent mammalian stem cells. The invention further provides methods for the stabilization of a neural cell in culture comprising contacting the neural cell with MEDII conditioned medium. Preferably, the stabilized neural cell is a neural progenitor cell.
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Citations
55 Claims
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1. A method of stabilizing a neural cell comprising:
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(a) providing a pluripotent mammalian cell;
(b) culturing the pluripotent mammalian cell to produce a neural cell; and
(c) contacting the neural cell with a MEDII conditioned medium for greater than 2 passages to thereby stabilize the neural cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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30. A method of stabilizing a neural cell comprising:
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(a) providing a mammalian neural cell; and
(b) contacting the neural cell with a MEDII conditioned medium for greater than 2 passages to thereby stabilize the neural cell. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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- 46. A composition comprising an isolated neural cell, wherein the cell expresses nestin, wherein the cell has been stabilized by contact with MEDII conditioned medium for greater than 2 passages, and wherein the cell can differentiate into more than one type of further differentiated neural cell.
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