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Methods for cloning mammals using reprogrammed donor chromatin or donor cells

  • US 20060212952A1
  • Filed: 05/24/2006
  • Published: 09/21/2006
  • Est. Priority Date: 12/22/2000
  • Status: Abandoned Application
First Claim
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1. A method of cloning a non-primate mammal, said method comprising the steps of:

  • (a) permeabilizing a non-primate mammalian cell, thereby generating a permeabilized cell having pores in its plasma membrane or a partial plasma membrane;

    (b) incubating said permeabilized cell in an extract from an oocyte under conditions that allow chromatin condensation and nuclear envelope breakdown of said permeabilized cell;

    (c) inserting said cell formed in step (b) into a nucleated or enucleated oocyte, thereby forming a reconstituted oocyte; and

    (d) transferring said reconstituted oocyte or an embryo formed from said reconstituted oocyte into the uterus of a host mammal under conditions that allow said reconstituted oocyte or said embryo to develop into a fetus.

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