Sensor platform, apparatus incorporating the platform and process using the platform

• US 20060216204A1
• Filed: 02/08/2006
• Published: 09/28/2006
• Est. Priority Date: 07/05/1999
• Status: Active Grant

• Current Assignee: Novartis Pharmaceuticals Corporation (Novartis Ag)
• Original Assignee: Novartis Pharmaceuticals Corporation (Novartis Ag)
• Inventors: Budach, Wolfgang Ernst Gustav, Neuschaefer, Dieter
• Granted Patent: US 7,820,106 B2
• Time in Patent Office: Days
• US Class Current: 422/82.80
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 20/00 Nanooptics, e.g. quantum optics or photonic crystals
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide

Abstract

A sensor platform for use in sample analysis comprises a substrate (30) of refractive index (n₁) and a thin, optically transparent layer (32) of refractive index (n₂) on the substrate, (n₂) is greater than (n₁). The platform incorporates one or multiple corrugated structures in the form of periodic grooves (31), (33), which defines one or more sensing areas each for one or more capture elements. The grooves are so profiled, dimensioned and oriented that when coherent light is incident on the platform it is diffracted into individual beams or diffraction order resulting in reduction of the transmitted beam and an abnormal high reflection of the incident light thereby creates an enhanced evanescent field at the surface of the or each sensing area. The amplitude of this field at the resonant condition is greater by an order of approximately 100 than the field of prior art platforms so that the luminescence intensity created from samples on the platform is also increased by a factor of 100. Also disclosed are an apparatus incorporating the platform and a method of using the platform. Further increases of amplitude have been detected by using light having a linear component which gives rise to TM excitation and/or irradiating the platform from the substrate side.

Claims

1-25. -25. (canceled)

26. A platform for use in sample analysis, said platform having one or more sensing areas or regions, each for receiving a capture element or elements which when the platform is irradiated with coherent light can interact to provide an indication of an affinity reaction, wherein each capture element includes two or more types of capture molecule.

30. The platform of claim 26, including an optically transparent substrate having a first refractive index (n₁), a thin, optically transparent layer, formed on one surface of the substrate, said layer having a second refractive index (n₂) which is greater than the first refractive index (n₁), said platform incorporating therein one or multiple corrugated structures comprising periodic grooves which define the one or multiple sensing regions, said grooves being configured such that coherent light incident on said platform is diffracted resulting in an abnormal high reflection of the incident light thereby generating an enhanced evanescent field at a surface of the one or multiple sensing regions;

wherein the enhanced evanescent field interacts with luminescent material on or in the vicinity of the surface of the one or more of the sensing regions so as to produce a detectable luminescent signal.

31. The platform of claim 30, wherein the light incident upon the platform has a polarization that is substantially perpendicular to the grooves in at least one sensing region so as to give rise to TM excitation in the at least one sensing region.

32. A process according to claim 31 wherein the light beam is incident onto the substrate side of the platform.

33. A process according to claim 31 wherein the light beam is incident onto the optically transparent layer side of the platform.

34. The platform of claim 30, wherein the light beam incident upon the platform has a polarization that is substantially parallel to the grooves in at least one sensing region so as to give rise to TE excitation in the at least one sensing region.

35. A process according to claim 34 wherein the light beam is incident onto the substrate side of the platform.

36. A process according to claim 34 wherein the light beam is incident onto the optically transparent layer side of the platform.

27-29. -29. (canceled)