Reconstitutable dried blood products

• US 20060216687A1
• Filed: 12/24/2003
• Published: 09/28/2006
• Est. Priority Date: 12/24/2002
• Status: Abandoned Application

• Current Assignee: Sinvent AS
• Original Assignee: Sinvent AS
• Inventors: Bjork, Peter, Eikevik, Trygve Magne, Strommen, Ingvald, Alves-Filho, Odilio, Bergslien, Ola
• Application Number:
  US10/540,520
• Publication Number:
  US 20060216687A1
• Time in Patent Office: Days
• US Class Current: 435/2
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0221 Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid

Abstract

The invention provides a process for the preparation of a dried particulate blood product the particles whereof comprise anuclear blood cells in a protective agent, said process comprising: obtaining a blood sample from a mammalian subject; adding an anticoagulant to said sample; concentrating the cells of said sample; recovering a concentrate containing anuclear blood cells from said sample; impregnating with said concentrate a particulate comprising a macromolecular protective material; drying the impregnated particulate at a temperature in the range of −20 to +120° C.; and, optionally, packaging the dried particular in sealed containers.

Claims

1. A process for the preparation of a dried particulate blood product the particles whereof comprise anuclear blood cells in a protective agent, said process comprising:

obtaining a blood sample from a mammalian subject;

adding an anticoagulant to said sample;

concentrating the cells of said sample;

recovering a concentrate containing anuclear blood cells from said sample;

impregnating with said concentrate a particulate comprising a macromolecular protective material;

drying the impregnated particulate at a temperature in the range of −

20 to +120°

C.; and

, optionally, packaging the dried particulate in sealed containers.

2. The process as claimed in claim 1, wherein said blood sample is obtained from a human subject.

3. The process as claimed in claim 1, wherein said macromolecular protective material contains a water-soluble macromolecular substance having a molecular weight above 2000 D.

4. The process as claimed in claim 1, wherein said macromolecular protective material contains macromolecules naturally occurring in the blood of the species from which the blood derives.

5. The process as claimed in claim 1, wherein said macromolecular protective material has a hemoglobin content of less than 1% wt relative to the hemoglobin content of erythrocytes.

6. The process as claimed in claim 1, wherein the drying step is effected at a temperature between 1 and 10°

C.

7. The process as claimed in claim 1, wherein the drying step involves fluidized bed drying.

8. A dried particulate blood product the particles whereof comprising anuclear blood cells in a macromolecular protective material.

11. A method of production of a transfusion liquid, said method comprising dispersing a dried particulate blood product according to claim 8 or claim 9 in a physiologically tolerable sterile aqueous solution and optionally treating the resulting dispersion to reduce the content therein of the protective material.

12. A kit comprising a first container containing a dried particulate blood product according to claim 8 or claim 9, and a second container containing a sterile physiologically tolerable aqueous reconstitution solution.

13. A kit as claimed in claim 12, wherein said first container is disposed in said second container and is openable whereby to release its contents into said solution.

9. A dried, reconstitutable, biological product comprising nucleus-containing eukaryotic cells in a macromolecular protective material.

10. A process for preparation of a dried, reconstitutable biological product which process comprises impregnating a particulate macromolecular protective material with a liquid containing nucleus containing eukaryotic cells and drying the impregnated particulate.