METHOD FOR DETERMINING THE GLUCOSE CONCENTRATION BY FLUORESCENCE POLARIZATION

• US 20060216773A1
• Filed: 03/23/2006
• Published: 09/28/2006
• Est. Priority Date: 03/23/2005
• Status: Active Grant

• Current Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: von der Eltz, Herbert, Hoenes, Joachim
• Granted Patent: US 7,439,080 B2
• Time in Patent Office: Days
• US Class Current: 435/14
• CPC Class Codes: G01N 2333/4724 Lectins
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose

Abstract

The invention generally relates to a method for in vivo glucose monitoring in which the glucose concentration is measured by means of fluorescence polarization by detecting the change in viscosity, caused by changes in the glucose concentration.

Claims

1. A method for in vivo glucose monitoring in an individual, the method comprising:

filling a container that is permeable to glucose and having an inner space with a mixture containing a glucose-binding lectin and a probe;

sealing the container;

operatively coupling the container with a sensor, wherein the sensor is for measuring the fluorescence polarization;

implanting the container and the operatively coupled sensor into the individual; and

measuring the glucose concentration by detecting a change in viscosity in the inner space of the container by means of fluorescence polarization.

2. The method according to claim 1, wherein the glucose-binding lectin is concanavalin A, dextran.

3. The method according to claim 1, wherein the probe comprises a fluorophore and a carrier.

4. The method according to claim 3, wherein a protein is used as the carrier.

5. The method according to claim 3, wherein the fluorophore is a long-lived fluorophore with a fluorescence lifetime of >

100 ns.

6. The method according to claim 1, wherein the container is made of a semipermeable membranes selected from a group consisting of cellulose, cellulose derivatives, polyamide, polyamide derivatives, polysulfones or polysulfone derivatives.

7. The method according to claim 1, wherein the sensor is a light guide capable of beaming light in or out.

8. The method according to claim 1, wherein the determination of the glucose concentration is carried out continuously.

9. The method according to claim 1, the molecular weight of the probe is adjusted such that the probe cannot diffuse out of the container.

10. A system for in vivo glucose monitoring in an individual using florescence polarization, the system comprising:

a container comprising a glucose-binding lectin, and a probe; and

a sensor operatively coupled to the container for measuring the glucose concentration using florescence polarization.

11. The system according to claim 10, wherein the glucose-binding lectin is concanavalin A, dextran.

12. The system according to claim 10, wherein the probe comprises a fluorophore and a carrier.

13. The system according to claim 12, wherein a protein is used as the carrier.

14. The system according to claim 12, wherein the fluorophore is a long-lived fluorophore with a fluorescence lifetime of >

100 ns.

15. The system according to claim 10, wherein the container is made of a semipermeable membranes selected from a group consisting of cellulose, cellulose derivatives, polyamide, polyamide derivatives, polysulfones or polysulfone derivatives.

16. The system according to claim 10, wherein the sensor is a light guide capable of beaming light in or out.

17. The system according to claim 10, wherein the determination of the glucose concentration is carried out continuously.

18. The system according to claim 10, the molecular weight of the probe is adjusted such that the probe cannot diffuse out of the container.