HYALURONAN SYNTHASE GENE AND USES THEREOF

• US 20060216808A1
• Filed: 06/05/2006
• Published: 09/28/2006
• Est. Priority Date: 07/01/1994
• Status: Active Grant

• Current Assignee: Kshama Kumari, Paul Deangelis, Paul H. Weigel
• Original Assignee: Kshama Kumari, Paul Deangelis, Paul H. Weigel
• Inventors: Weigel, Paul H., Kumari, Kshama, DeAngelis, Paul
• Granted Patent: US 7,153,677 B2
• Time in Patent Office: Days
• US Class Current: 435/193
• CPC Class Codes: A61K 39/102 Pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C08B 37/0072 Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: C12P 19/26 Preparation of nitrogen-containing carbohydrates
• CPC Class Codes: Y10S 435/836 Bacillus licheniformis
• CPC Class Codes: Y10S 435/839 Bacillus subtilis
• CPC Class Codes: Y10S 435/848 Escherichia

Abstract

The present invention relates to a recombinantly produced, enzymatically active hyaluronan synthase, wherein the recombinantly produced, enzymatically active hyaluronan synthase is a single protein that is a dual-action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA. The recombinantly produced, enzymatically active hyaluronan synthase may be produced from a hyaluronan synthase gene isolated from a microbial source, such as a streptococcal source.

Claims

1. A recombinantly produced, enzymatically active hyaluronan synthase, wherein the recombinantly produced, enzymatically active hyaluronan synthase is a single protein that is a dual- action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA.

2. The recombinantly produced, enzymatically active hyaluronan synthase of claim 1, wherein the recombinantly produced, enzymatically active hyaluronan synthase is produced from a hyaluronan synthase gene isolated from a microbial source.

3. The recombinantly produced, enzymatically active hyaluronan synthase of claim 1, wherein the recombinantly produced, enzymatically active hyaluronan synthase is produced from a hyaluronan synthase gene isolated from a streptococcal source.

4. An isolated, recombinantly produced, enzymatically active hyaluronan synthase, wherein the recombinantly produced, enzymatically active hyaluronan synthase is a single protein that is a dual-action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA.

5. The isolated, recombinantly produced, enzymatically active hyaluronan synthase of claim 4, wherein the isolated, recombinantly produced, enzymatically active hyaluronan synthase is produced from a hyaluronan synthase gene isolated from a microbial source.

6. The isolated, recombinantly produced, enzymatically active hyaluronan synthase of claim 4, wherein the isolated, recombinantly produced, enzymatically active hyaluronan synthase is produced from a hyaluronan synthase gene isolated from a streptococcal source.

7. A purified, recombinantly produced, enzymatically active hyaluronan synthase, wherein the recombinantly produced, enzymatically active hyaluronan synthase is a single protein that is a dual- action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA.

8. The purified, recombinantly produced, enzymatically active hyaluronan synthase of claim 7, wherein the isolated, recombinantly produced, enzymatically active hyaluronan synthase is produced from a hyaluronan synthase gene isolated from a microbial source.

9. The purified, recombinantly produced, enzymatically active hyaluronan synthase of claim 7, wherein the isolated, recombinantly produced, enzymatically active hyaluronan synthase is produced from a hyaluronan synthase gene isolated from a streptococcal source.

10. A recombinantly produced, enzymatically active hyaluronan synthase, wherein the recombinantly produced, enzymatically active hyaluronan synthase is a single protein that is a dual- action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA, and wherein the recombinantly produced, enzymatically active hyaluronan synthase is produced from a hyaluronan synthase gene isolated from a microbial source.

11. A recombinantly produced, enzymatically active hyaluronan synthase, wherein the recombinantly produced, enzymatically active hyaluronan synthase is a single protein that is a dual- action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA, and wherein the recombinantly produced, enzymatically active hyaluronan synthase is produced from a hyaluronan synthase gene isolated from a streptococcal source.