Flow path system provided with reaction section suited for the detection of hybridization, and hybridization detecting device making use of the flow path system

• US 20060216816A1
• Filed: 03/20/2006
• Published: 09/28/2006
• Est. Priority Date: 03/22/2005
• Status: Abandoned Application

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Ohnishi, Michihiro
• Application Number:
  US11/378,361
• Publication Number:
  US 20060216816A1
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: B01D 15/345 Perfusive chromatography
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/561 Tubes; Conduits in general F16L
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: G01N 30/02 Column chromatography

Abstract

A flow path system suitable for use in the detection of hybridization includes a capillary provided at a predetermined location thereof with a reaction section packed with beads. On the beads, a linker having a sequence of bases of the same type is immobilized, and a target nucleic acid complementarily bound to the sequence of the bases of the same type is held.

Claims

1. A flow path system comprising:

a capillary provided at a predetermined location thereof with a reaction section packed with beads;

wherein on said beads, a linker having a sequence of bases of the same type is immobilized and a target nucleic acid complimentarily bound to said sequence of said bases of the same type is held.

2. The flow path system according to claim 1, wherein in said reaction section, hybridization is allowed to proceed between a single-stranded segment of said target nucleic acid and a probe nucleic acid fed into said reaction section.

3. The flow path system according to claim 1, wherein said sequence of said bases of the same type in said linker is a polyT, and said target nucleic acid is a mRNA having a polyAtail segment.

4. The flow path system according to claim 3, wherein said mRNA is a mRNA available from lysis of cells, which have been injected into said flow path system, at a predetermined location in said flow path system.

5. The flow path system according to claim 1, wherein said probe nucleic acid is labeled with a fluorescent substance.

6. The flow path system according to claim 1, further comprising

a feeding-promoting section arranged in a region on a downstream side of said reaction section such that said feeding-promoting section can contribute to a speed-up in the feeding of each solution.

7. The flow path system according to claim 1, wherein said feeding-promoting section is packed with perfusion chromatography particles.

8. The flow path system according to claim 6, wherein said reaction section and its subsequent feeding-promoting section are repeatedly arranged in at least two combinations.

9. The flow path system according to claim 6, wherein a fraction of said probe nucleic acid, said fraction having taken no part in said hybridization, is trapped in said feeding-promoting section.

10. A multiple flow path system comprising

a plurality of flow path systems as defined in claim 1.

11. A hybridization detecting system comprising:

a flow path system as defined in claim 1;

and

a detection unit for detecting hybridization between said target nucleic acid, which exists in said reaction section of said flow path system, and a probe nucleic acid fed into said reaction section.

12. The hybridization detecting system according to claim 11, wherein said detection unit is provided at least with an excitation-light irradiating sub-unit for exciting a fluorescent substance labeled on the probe nucleic acid and a photodetector sub-unit for capturing excited fluorescence available from said reaction section.