Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof
First Claim
1. Isolated mammalian pluripotent embryonic-like stem cells (ELSCs) which (i) are capable of proliferating in an in vitro culture, (ii) maintain the potential to differentiate into cells of endoderm, mesoderm or ectoderm lineage in culture, and (iii) are capable of forming embryoid-like bodies when placed in suspension culture.
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Abstract
The present disclosure describes mammalian pluripotent embryonic-like stem cells (ELSCs) isolated from corneal limbal tissue, a non-embryonic tissue. The ELSCs of the present disclosure are capable of proliferating in an in vitro culture, maintain the potential to differentiate into cells of endoderm, mesoderm, and ectoderm lineage in culture, and are capable of forming embryoid-like bodies when placed in suspension culture. Thus, these cells possess multi-lineage differentiation potential and self-renewing capability. ELSCs may be a promising therapeutic tool, and may provide new therapeutic alternatives for various diseases, conditions, and injuries.
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32 Claims
- 1. Isolated mammalian pluripotent embryonic-like stem cells (ELSCs) which (i) are capable of proliferating in an in vitro culture, (ii) maintain the potential to differentiate into cells of endoderm, mesoderm or ectoderm lineage in culture, and (iii) are capable of forming embryoid-like bodies when placed in suspension culture.
- 7. Isolated mammalian pluripotent embryonic-like stem cells (ELSCs) which (i) are isolated from corneoscleral limbus, (ii) are capable of proliferating in an in vitro culture, and (iii) maintain the potential to differentiate into lineage-committed endodermal, ectodermal, or mesodermal cells.
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12. A method of isolating a population of mammalian pluripotent embryonic-like stem cells (ELSCs), comprising the steps of:
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(a) isolating corneal limbal tissue from a donor;
(b) culturing the corneal limbal tissue to expand corneal limbal cells in culture; and
(c) isolating a population of pluripotent ELSCs from the cultured corneal limbal cells by sorting the corneal limbal cells to select for one or more undifferentiated cell-specific surface markers. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification