Amnion-derived cell compositions, methods of making and uses thereof
First Claim
1. A substantially purified population of amnion-derived cells that is negative for expression of the protein markers CD90 and CD117.
2 Assignments
0 Petitions
Accused Products
Abstract
The invention is directed to substantially purified amnion-derived cell populations, compositions comprising the substantially purified amnion-derived cell populations, and to methods of creating such substantially purified amnion-derived cell populations, as well as methods of use. The invention is further directed to antibodies, in particular, monoclonal antibodies, that bind to amnion-derived cells or, alternatively, to one or more amnion-derived cell surface protein markers. The invention is further directed to methods for producing the antibodies, methods for using the antibodies, and kits comprising the antibodies.
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Citations
55 Claims
- 1. A substantially purified population of amnion-derived cells that is negative for expression of the protein markers CD90 and CD117.
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12. A method of obtaining a substantially purified population of amnion-derived cells, comprising:
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a) providing a population of amnion-derived cells;
b) contacting the cells with (i) one or more antibodies selected from the group consisting of anti-CD105, anti-CD90, anti-CD117, anti-CD140b, anti-CD34, anti-CD44, and anti-CD45, antibodies; and
(ii) one or more antibodies selected from the group consisting of anti-CD29, anti-CD9, anti-CD10, anti-CD26, anti-CD71, anti-CD166, anti-CD227, anti-EGF-R, anti-SSEA-4, and anti-HLA-G antibodies; and
c) separating the cells that do not bind to the antibodies of (i) from the cells that do bind to the antibody of (i) and separating the cells that do not bind to the antibodies of (ii) from the cells that do bind to the antibody of (ii) such that a substantially purified population of amnion-derived cells that do not bind to the antibodies of (i) and do bind to the antibody of (ii) is obtained.
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- 23. A method for promoting accelerated wound healing in an injured patient in need thereof comprising administering to the patient one or more compositions selected from the group consisting of placental-derived cells, conditioned media derived from placental-derived cells, placental-derived cell lysates, and placental-derived cell products.
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26. A cosmetic preparation comprising one or more compositions selected from the group consisting of placental-derived cells, conditioned media derived from placental-derived cells, placental-derived cell lysates, and placental-derived cell products.
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27. A method for treating hearing loss in a patient in need thereof comprising administering to the patient one or more compositions selected from the group consisting of placental-derived cells, conditioned media derived from placental-derived cells, placental-derived cell lysates, and placental-derived cell products.
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47. The composition of claim 4469, wherein the identifying characteristics of endoderm are protein expression of HNF1α
- , HNF1β
, HNF4α
, HNF-6, Foxa2 and PDX1 and wherein the cells, further optionally express any one or more of the protein markers Sox17, Cerberus, Hesx1, LeftyA, Otx1 or Otx2.
- , HNF1β
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49. An in vivo method for inducing differentiation of resident pancreatic cells into islet cells comprising:
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a) introducing factors into the pancreas of a subject; and
b) allowing the introduced factors to prime the resident pancreatic cells such that the cells are induced to differentiate into islet progenitor cells and/or islet cells.
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Specification