Methods of nucleic acid analysis
First Claim
1. A method of characterising a target base in a sample nucleic acid, which method comprises:
- (a) contacting the sample nucleic acid with an oligonucleotide primer under conditions which allow hybridisation of the oligonucleotide to the sample nucleic acid, said oligonucleotide primer being labelled with a fluorophore;
(b) contacting the sample nucleic acid with a deoxynucleotide or dideoxynucleotide which is labelled with a fluorophore, under conditions which allow extension of the oligonucleotide primer through incorporation of the labelled nucleotide; and
(c) measuring the fluorescence emitted by one or both of the fluorophores.
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Abstract
The present invention relates to a method of characterising a target base in a sample nucleic acid, which method comprises: (a) contacting the sample nucleic acid with an oligonucleotide primer under conditions which allow hybridisation of the oligonucleotide to the sample nucleic acid, said oligonucleotide primer being labelled with a fluorophore; (b) contacting the sample nucleic acid with a deoxynucleotide or dideoxynucleotide which is labelled with a fluorophore, under conditions which allow extension of the oligonucleotide primer through incorporation of the labelled nucleotide; and (c) measuring the fluorescence emitted by one or both of the fluorophores.
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Citations
21 Claims
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1. A method of characterising a target base in a sample nucleic acid, which method comprises:
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(a) contacting the sample nucleic acid with an oligonucleotide primer under conditions which allow hybridisation of the oligonucleotide to the sample nucleic acid, said oligonucleotide primer being labelled with a fluorophore;
(b) contacting the sample nucleic acid with a deoxynucleotide or dideoxynucleotide which is labelled with a fluorophore, under conditions which allow extension of the oligonucleotide primer through incorporation of the labelled nucleotide; and
(c) measuring the fluorescence emitted by one or both of the fluorophores. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification