Mutant DNA polymerases and methods of use
First Claim
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1. A mutant DNA polymerase comprising an Asn residue at amino acid 543 and a 5′
- -3′
exonuclease activity reducing mutation, wherein the positions of amino acids of the mutant DNA polymerase are defined with respect to Taq DNA polymerase.
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Abstract
The present invention provides mutant DNA polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a DNA polymerase of the invention.
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Citations
31 Claims
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1. A mutant DNA polymerase comprising an Asn residue at amino acid 543 and a 5′
- -3′
exonuclease activity reducing mutation, wherein the positions of amino acids of the mutant DNA polymerase are defined with respect to Taq DNA polymerase. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification