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Method for nucleic acid analysis

  • US 20060223087A1
  • Filed: 01/31/2006
  • Published: 10/05/2006
  • Est. Priority Date: 03/30/2005
  • Status: Abandoned Application
First Claim
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1. A method for nucleic acid analysis to analyze one or more noncomplementary sites in a double-stranded nucleic acid sample, comprising the steps of:

  • obtaining nucleic acid fragments by cleaving the one or more noncomplementary sites with a single strand-specific endonuclease;

    hybridizing at least one of the obtained nucleic acid fragments to a probe having a nucleotide sequence that is partially or totally identical to either one strand of the double-stranded nucleic acid sample;

    performing an extension reaction from the nucleic acid fragment hybridized to the probe; and

    optically detecting pyrophosphate generated by the extension reaction.

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