Multiplex fluorescence detection device having removable optical modules
First Claim
26. A detection system comprising. a data acquisition device;
- and a detection device coupled to the data acquisition device, wherein the detection device comprises;
a motor to rotate a disk having a plurality of process chambers each holding a respective sample and one or more fluorescent dyes;
a plurality of optical modules, and a housing having a plurality of locations adapted to receive the optical modules;
wherein each of the optical modules includes an optical channel having a light source selected for a different one of the dyes and a lens to capture fluorescent light emitted from the disk.
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Abstract
Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules. Each of the removable optical modules is optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector.
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Citations
63 Claims
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26. A detection system comprising.
a data acquisition device; - and
a detection device coupled to the data acquisition device, wherein the detection device comprises;
a motor to rotate a disk having a plurality of process chambers each holding a respective sample and one or more fluorescent dyes;
a plurality of optical modules, and a housing having a plurality of locations adapted to receive the optical modules;
wherein each of the optical modules includes an optical channel having a light source selected for a different one of the dyes and a lens to capture fluorescent light emitted from the disk. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A method comprising:
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rotating a disk having a plurality of process chambers each having one or more species that emit fluorescent light at different wavelengths;
exciting the disk with a plurality of light beams to produce a plurality of emitted fluorescent light beams; and
capturing the fluorescent light beams with a plurality of different removable optical modules, wherein the removable optical modules are optically configured for the different wavelengths and contained within a housing. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47)
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48. A removable optical module for a detection device comprising:
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an optical channel having a light source selected for a different one of a plurality of fluorescent dyes and a lens to capture fluorescent light emitted from a disk;
a set of one or more optical module electrical contacts for electronically coupling to a set of one or more housing electrical contacts within a housing; and
an attachment mechanism to attach the optical module to the housing. - View Dependent Claims (49, 50, 51, 52)
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53. A removable dual optical channel optical module for a detection device comprising:
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a first optical channel having a light source selected for a first fluorescent dye and a lens to capture fluorescent light of a first wavelength emitted from a disk;
a second optical channel having a light source selected for a second fluorescent dye and a lens to capture fluorescent light of a second wavelength emitted from the disk;
a set of one or more optical module electrical contacts for electronically coupling to a set of one or more housing electrical contacts within a housing; and
an attachment mechanism to attach the optical module to the housing. - View Dependent Claims (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 54, 55, 56)
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54-1. The optical module of claim 53, further comprising a first optical output port for coupling to a first leg of a fiber optic bundle attached to a detector and a second optical output port for coupling to a second leg of a fiber optic bundle attached to the detector.
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57. A removable optical module having an optical channel and a laser source for a detection device comprising:
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an optical channel having a light source selected for a different one of a plurality of fluorescent dyes and a lens to capture fluorescent light emitted from a disk;
a laser source;
a set of one or more optical module electrical contacts for electronically coupling to a set of one or more housing electrical contacts within a housing; and
an attachment mechanism to attach the optical module to the housing. - View Dependent Claims (58, 59, 60, 61, 62)
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63. A detection device comprising:
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a motor to rotate a disk having a plurality of process chambers each holding a respective sample and one or more fluorescent dyes;
one or more optical modules, and a housing having a location adapted to receive one optical module;
wherein each of the optical modules includes an optical channel having a light source selected for a different one of the dyes and a lens to capture fluorescent light emitted from the disk.
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Specification