Method for manufacturing an enzymatic electrochemical-based sensor

• US 20060226007A1
• Filed: 04/28/2005
• Published: 10/12/2006
• Est. Priority Date: 04/12/2005
• Status: Abandoned Application

• Current Assignee: LifeScan Scotland Limited (Johnson & Johnson)
• Original Assignee: LifeScan Scotland Limited (Johnson & Johnson)
• Inventors: Lillie, Geoffrey, Rodgers, James Iain, MacDonald, Michael, Liu, Zuifang, MacLennan, Margaret, Moffat, James, McNeilage, Alan Watson
• Application Number:
  US11/118,894
• Publication Number:
  US 20060226007A1
• Time in Patent Office: Days
• US Class Current: 204/403.140
• CPC Class Codes: C09D 11/52 Electrically conductive inks
• CPC Class Codes: C12Q 1/004 mediator-assisted

Abstract

A method for manufacturing a portion of an enzymatic electrochemical-based sensor includes applying a water-miscible conductive ink to a substrate of an enzymatic electrochemical-based sensor. The water-miscible conductive ink includes a conductive material, an enzyme, a mediator, and a binding agent (that becomes operatively water-insoluble upon drying) formulated as a water-miscible aqueous-based dispersion. The method further includes the drying the water-miscible conductive ink to form a conductive layer on the substrate that includes an operatively water-insoluble binding agent.

Claims

1. A method for manufacturing a portion of an enzymatic electrochemical-based sensor, the method comprising:

applying a water-miscible conductive ink to a substrate of an enzymatic electrochemical-based sensor, wherein the water-miscible conductive ink includes;

a conductive material;

an enzyme;

a mediator; and

a binding agent,

wherein the conductive material, enzyme, mediator, and binding agent are formulated as a water-miscible aqueous-based dispersion and wherein the binding agent becomes operatively water-insoluble upon drying; and

drying the water-miscible conductive ink to form a conductive layer on the substrate that includes an operatively water-insoluble binding agent.

2. The method of claim 1, wherein the applying step is accomplished using a screen printing technique.

3. The method of claim 1, wherein the drying step is accomplished at a temperature of about 75°

C. for a time period of about 20 minutes.

4. The method of claim 1, wherein the drying step immobilizes the water-miscible conductive ink to the substrate, thus forming the conductive layer.

5. The method of claim 1, wherein the applying step applies a water-miscible conductive ink that includes a polymeric mediator.

6. The method of claim 1, wherein the drying step forms a conductive layer that serves as an electrode of the enzymatic electrochemical-based sensor.

7. The method of claim 6, wherein the drying step forms a conductive layer that serves as a working electrode of the enzymatic electrochemical-based sensor.

8. The method of claim 1, wherein the applying step applies a water-miscible conductive ink wherein the enzyme is a glucose oxidizing enzyme.

9. The method of claim 1, wherein the applying step applies a water-miscible conductive ink formulated as a uniform dispersion.

10. The method of claim 9, wherein the drying step forms a conductive layer containing uniformly distributed enzyme, mediator and conductive material.

11. The method of claim 1, wherein the applying step applies a water-miscible conductive ink containing conductive material that includes finely divided conductive particles with a size of less than 100 microns.