Blood collection and measurement apparatus

• US 20060228258A1
• Filed: 04/12/2005
• Published: 10/12/2006
• Est. Priority Date: 04/12/2005
• Status: Abandoned Application

• Current Assignee: ChroMedX Inc.
• Original Assignee: ChroMedX Inc.
• Inventors: Samsoondar, James
• Application Number:
  US11/103,619
• Publication Number:
  US 20060228258A1
• Time in Patent Office: Days
• US Class Current: 422/82.50
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: G01N 2021/0314 Double pass, autocollimated path
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/0382 Frustoconical, tapered cell
• CPC Class Codes: G01N 2021/054 Bubble trap; Debubbling
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/11 Filling or emptying of cuvettes
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

Some embodiments of the invention provide one apparatus that is suitable for both the collection and measurement of a blood sample. Once a blood sample is drawn into such an apparatus the blood sample can be analyzed, without having to transfer any portion of the blood sample into another vessel. Also, in some very specific embodiments, the apparatus is provided with an optical chamber that is specifically designed to reduce the average attenuation of electromagnetic radiation (EMR) due to scattering of EMR by the red blood cells in a blood sample, without having to hemolyze the red blood cells by using sound waves or by adding reagents to the blood sample. Moreover, as a result of the time saved by using a single apparatus for blood sample collection and measurement, the addition of an anticoagulant is not required to prevent clotting. Moreover, in such embodiments the optical chamber is designed to spread blood into a thin film, thereby reducing the incidences of trapped air bubbles in the collected blood sample in the optical chamber. Instead air bubbles are easily pushed through the optical chamber and guided out of the apparatus through a vent. Optionally, at least one biosensor may be provided within a second flow path in the apparatus.

Claims

1. A blood sample collection and measurement apparatus comprising:

a housing having a side dimension and a depth dimension orthogonal to the side dimension,

an inlet transition cavity within the housing for receiving blood to be analyzed;

an optical chamber, within the housing, for receiving the blood from the inlet transition cavity, the optical chamber having at least one optical window for viewing the blood and an optical chamber depth extending from the at least one optical window parallel to the depth dimension;

an overflow chamber, within the housing, for receiving blood from the optical chamber; and

an outlet vent, in the housing and fluidly connected to the overflow chamber, to provide an outflow path for air.

2. A blood sample collection and measurement apparatus according to claim 1, wherein the inlet transition cavity has an inlet transition cavity depth parallel to the depth dimension and an inlet transition cavity width parallel to the side dimension.

3. A blood sample collection and measurement apparatus according to claim 1, wherein the average optical chamber depth is in the approximate range of 0.02 mm to 0.2 mm.

4. The fluid collection device as defined in claim 1 wherein the average optical chamber depth is less than 0.1 mm.

5. A blood sample collection and measurement apparatus according to claim 1, wherein the inlet transition cavity comprises a tapered transition region bordering the optical chamber, wherein within the tapered transition region the inlet transition cavity width increases toward the optical region and the inlet transition cavity depth diminishes toward the optical chamber.

6. A blood sample collection and measurement apparatus according to claim 5, wherein the inlet transition cavity width substantially equals an optical chamber width at a juncture of the transition region and the optical chamber, and the inlet transition cavity depth substantially equals the optical chamber depth at the juncture of the transition region with the optical chamber

7. A blood sample collection and measurement apparatus according to claim 1, wherein the inlet transition cavity has a respective inlet transition cavity volume and the optical chamber has an optical chamber volume, the optical chamber volume being less than half the inlet transition cavity volume.

8. A blood sample collection and measurement apparatus according to claim 7, wherein the optical chamber volume is less than one-quarter the inlet transition cavity volume.

9. A blood sample collection and measurement apparatus according to claim 8, wherein a sum of the overflow chamber volume, the optical chamber volume and the inlet transition cavity volume is less than 30 micro-liters.

10. A blood sample collection and measurement apparatus according to claim 9, wherein the sum of the overflow chamber volume, the optical chamber volume and the inlet transition cavity volume is less than 15 micro-liters, and the optical chamber volume is less than 2 micro-liters.

11. A blood sample collection and measurement apparatus according to claim 1, wherein the overflow chamber has an overflow chamber volume at least equal to the optical chamber volume.

12. A blood sample collection and measurement apparatus according to claim 1, wherein the overflow chamber has a tapered region, and within the tapered region, an overflow chamber depth increases away from the optical chamber and toward the vent such that the overflow chamber depth exceeds 2 mm before the outlet vent.

13. A blood sample collection and measurement apparatus according to claim 1 further comprising a visible fill line for indicating a total amount of the fluid received into the apparatus, the optical chamber and the overflow chamber.

14. A blood sample collection and measurement apparatus according to claim 1 further comprising a reflective coating on a wall-portion of the optical chamber.

15. A blood sample collection and measurement apparatus according to claim 1, wherein the inlet transition cavity branches into two independent flow paths, the two independent flow paths comprising a first flow path including the optical chamber and terminating at the outlet vent, and a second flow path including a capillary tube terminating at another outlet vent and at least one biosensor arranged adjacent to the capillary tube.

16. A blood sample collection and measurement apparatus as defined in claim 15 further comprising a cap for covering an opening into the inlet transition cavity, the cap having a plunger for exerting pressure on blood within the apparatus.

17. A blood sample collection and measurement apparatus according to claim 15, wherein at least one of the first and second flow paths includes a capillary break for slowing the inflow of blood.

18. A blood sample collection and measurement apparatus according to claim 15, wherein the second flow path includes a capillary break for slowing the inflow of calibration fluid.

19. A blood sample collection and measurement apparatus according to claim 15 further comprising a plunger for exerting pressure on the blood within the apparatus.

20. A blood sample collection and measurement apparatus according to claim 15 further comprising a calibration reservoir containing a calibration fluid and having a release means for releasing the calibration fluid into the second flow path for measurement by the at least one biosensor, the calibration fluid having at least one known property for measurement by the at least one biosensor.

21. A blood sample collection and measurement apparatus as defined in claim 20 wherein the calibration reservoir is flexible such that pressure can be provided to the calibration fluid within the calibration reservoir, and the release means comprises a weakened wall portion of the calibration reservoir, the weakened wall pressure being breakable by fluid pressure in the calibration fluid to release the calibration fluid into the second flow path.