System and method for electronic sensing of biomolecules

• US 20060228723A1
• Filed: 10/25/2005
• Published: 10/12/2006
• Est. Priority Date: 01/16/2002
• Status: Abandoned Application

• Current Assignee: Nanomix Incorporated (CJY Holdings Ltd.)
• Original Assignee: Nanomix Incorporated (CJY Holdings Ltd.)
• Inventors: Bradley, Keith, Gruner, George, Tu, Eugene, Gabriel, Jean-Christophe P., Valcke, Christian, Star, Alexander
• Application Number:
  US11/259,414
• Publication Number:
  US 20060228723A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0663 Whole sensors
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 2300/0893 having a very large number of wells, microfabricated wells
• CPC Class Codes: B01L 2300/0896 Nanoscaled
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 3/50857 using arrays or bundles of open capillaries for holding samples
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/551 the carrier being inorganic
• CPC Class Codes: G01N 33/566 using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
• CPC Class Codes: G01N 33/582 with fluorescent label

Abstract

A nanoelectronic device is combined with a cellular membrane component to provide a sensor for biomolecules or to provide information about the structure of the membrane. The nanoelectronic device may comprise a network of randomly-oriented nanotubes, or other nanostructure, arranged on a substrate with adjacent electrodes so as to operate as a field-effect transistor sensor or as a capacitive sensor. A cellular membrane is disposed over the nanostructure element.

Claims

1. A nanoelectronic device, comprising:

a substrate;

at least one nanostructure disposed adjacent the substrate;

at least a first electrode arranged adjacent the substrate and in electrical communication with the nanostructure; and

at least a portion of a biological cell engaged with the nanoelectronic device, so as render the nanoelectronic device electrically responsive to a biomolecule analyte.

2. The nanoelectronic device of claim 1, wherein the at least one nanostructure comprises at least one carbon nanotube.

3. The nanoelectronic device of claim 1, wherein the at least one nanostructure comprises a network of nanotubes.

4. The nanoelectronic device of claim 1, wherein the nanoelectronic device further comprises at least a second electrode arranged adjacent the substrate and in electrical communication with the nanostructured element.

5. The nanoelectronic device of claim 1, wherein the nanoelectronic device further comprises at least a gate electrode disposed to influence an electrical property of the nanoelectronic device.

6. The nanoelectronic device of claim 1, wherein the nanoelectronic device further comprises at least a gate electrode disposed to influence an electrical property of the nanoelectronic device, and wherein the nanoelectronic device is electrically responsive to an electrical property of the at least one nanostructure under the influence of a gate voltage, the electrical property selected from the group consisting of transconductance and resistance.

7. The nanoelectronic device of claim 1, wherein the nanoelectronic device is electrically responsive to a capacitance of the least one nanostructured element.

8. The nanoelectronic device of claim 1, wherein the nanoelectronic device is configured to engage the at least a portion of a biological cell in an aqueous medium, and wherein the nanoelectronic device further comprises a reference electrode in electrical communication with the aqueous medium.

9. The nanoelectronic device of claim 1, wherein the at least a portion of a biological cell comprises an intact cell.

10. The nanoelectronic device of claim 1, wherein the at least a portion of a biological cell comprises a cell membrane.

11. The nanoelectronic device of claim 1, wherein the nanoelectronic device is configured to engage the at least a portion of a biological cell by direct contact of the nanostructure with the at least a portion of a biological cell.

12. The nanoelectronic device of claim 1, wherein the nanoelectronic device is configured to engage the at least a portion of a biological cell by the interaction of at least one intermediate functional molecule associated with the nanoelectronic device and the at least a portion of a biological cell.

13. The nanoelectronic device of claim 1, wherein the nanoelectronic device is configured to engage the at least a portion of a biological cell by the interaction of at least one intermediate functional molecule associated with the nanoelectronic device and the at least a portion of a biological cell, and wherein the at least one intermediate functional molecule comprises a binding species specific to a cell membrane structure.

14. The nanoelectronic device of claim 1, wherein the nanoelectronic device is configured to engage the at least a portion of a biological cell by interaction of the at least one nanostructure with a cytoplasmic side of a cell membrane.

15. The nanoelectronic device of claim 1, wherein the nanoelectronic device is configured to engage the at least a portion of a biological cell by the interaction of the at least one nanostructure with a extracellular side of a cell membrane.

16. The nanoelectronic device of claim 1, wherein the nanoelectronic device is configured to engage the at least a portion of a biological cell by the interaction of at least one intermediate functional molecule associated with the nanoelectronic device and the at least a portion of a biological cell, and wherein the at least one intermediate functional molecule includes a species enhancing a dipole effect of the engaging of the at least a portion of a biological cell.

17. The nanoelectronic device of claim 1, wherein the at least a portion of a biological cell includes a cell membrane of Halobacterium salinarum.

18. The nanoelectronic device of claim 1, further comprising an electrical measurement circuit in communication with the nanoelectronic device, the electrical measurement circuit measuring an electrical response of the nanoelectronic device.

19. The nanoelectronic device of claim 18, wherein the nanoelectronic device further comprises at least a gate electrode disposed to influence an electrical property of the nanoelectronic device, the electrical property selected from the group consisting of transconductance and resistance.

20. The nanoelectronic device of claim 19, wherein the electrical response comprises a change in the electrical property relative to a varying gate voltage applied by the gate electrode, the response selected from the group consisting of:

a change in a threshold of the electrical property, and a change in a hysteresis of the electrical property.