Light-controlled electrokinetic assembly of particles near surfaces
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Abstract
A method and apparatus for the manipulation of colloidal particles and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.
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Citations
142 Claims
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1-113. -113. (canceled)
- 114. A bead array comprising a planar assembly of encoded, oligonucleotide-bearing beads in a designated area on a substrate, wherein said encoded beads comprise a plurality of unique capture oligonucleotides and have n diameter of from 2 microns to 20 microns.
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115. A bead array comprising a planar assembly of encoded, oligonucleotide-bearing beads in a designated area on a substrate wherein said encoded beads comprise a plurality of unique capture oligonucleotides and have a diameter of up to 10 microns, and wherein differently-encoded beads are randomly distributed n said assembly.
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116. A bead array comprising a planar assembly of encoded, oligonucleotide-bearing beads in a designated area on a substrate, wherein said encoded beads comprise a plurality of unique capture oligonucleotides and have a diameter of about 1 micron and wherein differently-encoded beads are randomly distributed in said assembly.
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117. A bead array comprising a planar assembly of encoded, oligonucleotide-bearing beads, wherein said beads (a) are encoded by oligonucleotides, (b) comprise a plurality of unique capture oligonucleotides, (c) have a range of diameters of (i) from 2 microns to 20 microns or (ii) from several hundred Angstrom;
- up to 10 microns, wherein said beads are and wherein differently-encoded beads are randomly distributed within the planar assembly, and wherein said planar assembly is located in a designated area on a substrate.
- View Dependent Claims (118, 119, 120)
Specification