Adipose tissue-derived adult stem cells for the repair of articular cartilage fractures and uses thereof

• US 20060228796A1
• Filed: 06/08/2006
• Published: 10/12/2006
• Est. Priority Date: 08/19/1999
• Status: Abandoned Application

• Current Assignee: Cognate BioServices, Inc.
• Original Assignee: Artecel Inc.
• Inventors: Kolkin, Jon, Gimble, Jeffrey M.
• Application Number:
  US11/450,213
• Publication Number:
  US 20060228796A1
• Time in Patent Office: Days
• US Class Current: 435/325
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2506/1384 from adipose-derived stem cells [ADSC], from adipose stromal stem cells
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue
• CPC Class Codes: C12N 5/0655 Chondrocytes; Cartilage

Abstract

The invention provides cells, methods and compositions based upon the use of adipose tissue-derived adult stem cells in the repair of articular cartilage fractures or defects. The invention is useful in providing a treatment of articular cartilage fractures in a clinical setting.

Claims

1. A composition comprising an isolated adipose tissue-derived adult stem cell that can differentiate into a chondrocyte in combination with a viscous, biocompatible liquid material implanted into a host, wherein the host is in need of articular cartilage repair.

2. The composition of claim 1, wherein the biocompatible liquid is capable of gelling at body temperature.

3. The composition of claim 2, wherein the biocompatible liquid is selected from the group consisting of alginate, collagen, fibrin, hyaline, or plasma.

4. The composition of claim 1 in combination with a malleable, three dimensional matrix capable of filling an irregular cartilage defect.

5. The composition of claim 4, wherein the matrix is selected from the group consisting of polyglycolic-polylactic acid, poly-glycolic acid, poly-lactic acid, or suture-like material.

9. The composition of claim 1, wherein the cell is human.

10. The composition of claim 1, wherein the cell is modified with a nucleic acid.

6. A composition comprising an isolated adipose tissue-derived adult stem cell that can differentiate into a chondrocyte and implanted into host combined with a malleable, three dimensional matrix capable of filling an irregular cartilage defect and a solid phase, biocompatible material of sufficient structural integrity to serve as an anchor within the cancellous bone underlying the articular cartilage defect.

7. The composition of claim 6, wherein the biocompatible material comprises an isolated adipose tissue-derived stem cell that can differentiate into an osteoblast.

8. The composition of claim 7, wherein the isolated adipose tissue-derived stem cell is grown in a matrix in vitro prior to implantation.

11. A method of treating an articular cartilage defect in a host comprising implanting an isolated adipose tissue-derived adult stem cell that can differentiate into a chondrocyte.

12. The method of claim 11, wherein the isolated adipose tissue-derived adult stem cell is in combination with a viscous, biocompatible liquid material.

13. The method of claim 12, wherein the biocompatible liquid is capable of gelling at body temperature.

14. The method of claim 13, wherein the biocompatible liquid is selected from the group consisting of alginate, collagen, fibrin, hyaline, or plasma.

15. The method of claim 13 further comprising a malleable, three dimensional matrix capable of filling an irregular cartilage defect.

16. The method of claim 15, wherein the matrix is selected from the group consisting of polyglycolic-polylactic acid, poly-glycolic acid, poly-lactic acid, or suture-like material.

17. A method of treating an articular cartilage defect in a host comprising implanting a composition comprising an isolated adipose tissue-derived adult stem cell that can differentiate into a chondrocyte, a malleable, three dimensional matrix capable of filling an irregular cartilage defect and a solid phase, biocompatible material of sufficient structural integrity to serve as an anchor within the cancellous bone underlying the articular cartilage defect.

18. The method of claim 17 wherein the biocompatible material comprises an isolated adipose tissue-derived stem cell that can differentiate into an osteoblast.

19. The method of claim 18 wherein the isolated adipose tissue-derived stem cell that can differentiate into an osteoblast is grown in an matrix in vitro prior to implantation.