Microarrays displaying encoded molecules

• US 20060234231A1
• Filed: 06/20/2003
• Published: 10/19/2006
• Est. Priority Date: 06/20/2001
• Status: Abandoned Application

• Current Assignee: Nuevolution A/S (Amgen Inc.)
• Original Assignee: Nuevolution A/S (Amgen Inc.)
• Inventors: Pedersen, Henrik, Freskgard, Per-Ola
• Application Number:
  US10/518,056
• Publication Number:
  US 20060234231A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C07D 405/04 directly linked by a ring-member-to-ring-member bond
• CPC Class Codes: C07H 19/06 Pyrimidine radicals
• CPC Class Codes: C07H 19/10 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C07H 19/16 Purine radicals
• CPC Class Codes: C07H 19/20 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07H 23/00 Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12 esters with inorganic acids C07H11/00; metal salts, see parent compounds
• CPC Class Codes: C12N 15/1068 Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
• CPC Class Codes: C12P 19/34 Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• CPC Class Codes: C40B 40/00 Libraries per se, e.g. arrays, mixtures

Abstract

Disclosed is a microarray comprising a plurality of single stranded nucleic acid probes immobilized in discrete areas of a solid support, said probes being hybridised to a library of complexes, wherein each complex comprises an encoded molecule and a template which codes for said molecule, said template comprising a number of codons which codes for chemical entities which upon reaction form a reaction product which at least partly form part of the encoded molecule.

Claims

1. A microarray comprising a plurality of single stranded nucleic acid probes immobilized in discrete areas of a solid support, said probes being hybridised to a library of complexes, wherein each complex comprises an encoded molecule and a template which codes for said molecule, said template comprising a number of codons which codes for chemical entities which upon reaction form a reaction product which at least partly form part of the encoded molecule.

2. a microarray according to claim 1, wherein the chemical entities are precursors for a structural unit appearing in the encoded molecule.

3. A microarray according to claim 1, wherein the chemical entities are transferred to the nascent encoded molecule by a building block, which further comprises an anti-codon.

4. A microarray according to claim 3, wherein the information of the anti-codon is transferred in conjunction with the chemical entity to the nascent complex.

5. A microarray according to claim 1, wherein the chemical entities are reacted without enzymatic interaction.

6. A microarray according to claim 1, wherein the template comprises two or more codons.

7. A microarray according to claim 1, wherein the nucleic acid probe of the array is hybridised to a template through an adapter oligonucleotide having a sequence complementing the probe as well as the template.

9. A method for identifying an encoded molecule having a preselected property, comprising the steps of

i) providing the microarray according to claim 1

ii) adding a biological sample containing target molecules,

iii) washing non-bound material off, and

iv) detecting any bound material in each spot.

11. A microarray according to claim 2, wherein the chemical entities are transferred to the nascent encoded molecule by a building block, which further comprises an anti-codon.

12. A microarray according to claim 11, wherein the information of the anti-codon is transferred in conjunction with the chemical entity to the nascent complex.

13. A microarray according to claim 2, wherein the chemical entities are reacted without enzymatic interaction.

14. A microarray according to claim 2, wherein the template comprises two or more codons.

15. A microarray according to claim 2, wherein the nucleic acid probe of the array is hybridised to a template through an adapter oligonucleotide having a sequence complementing the probe as well as the template.

16. A microarray according to claim 11, wherein the chemical entities are reacted without enzymatic interaction.

17. A microarray according to claim 11, wherein the template comprises two or more codons.

18. A microarray according to claim 11, wherein the nucleic acid probe of the array is hybridised to a template through an adapter oligonucleotide having a sequence complementing the probe as well as the template.

19. A microarray according to claim 3, wherein the chemical entities are reacted without enzymatic interaction.

20. A microarray according to claim 3, wherein the template comprises two or more codons.

21. A microarray according to claim 3, wherein the nucleic acid probe of the array is hybridised to a template through an adapter oligonucleotide having a sequence complementing the probe as well as the template.

8. A method for preparing a microarray displaying a library of encoded molecules, wherein an oligonucleotide microarray comprising a plurality of single stranded nucleic acid probes immobilized in discrete areas of a solid support is mixed under conditions which allows for specific hybridisation with a library of complexes, each of said complexes comprising an encoded molecule and a template which codes for said molecule, said template comprising a number of codons which codes for chemical entities which upon reaction form a reaction product which at least partly form part of the encoded molecule.

10. (canceled)